| 张倩璐,李祥,王波,胡洁,旷昕.二氢杨梅素对丙泊酚麻醉引起的大鼠认知功能障碍的影响及机制.[J].中南医学科学杂志.,2019,(1):7-11. |
| 二氢杨梅素对丙泊酚麻醉引起的大鼠认知功能障碍的影响及机制 |
| Effects of dihydromyricetin on vognitive impairment induced by propofol anesthesia in rats |
| 投稿时间：2018-10-24 修订日期：2018-12-20 |
| DOI：10.15972/j.cnki.43-1509/r.2019.01.002 |
| 中文关键词: 二氢杨梅素 丙泊酚 认知功能障碍 小胶质细胞极化 一氧化氮合酶 精氨酸酶1 |
| 英文关键词:dihydromyricetin propofol cognitive dysfunction microglia polarization nitric oxide synthase arginase 1 |
| 基金项目: |
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| 中文摘要: |
| 二氢杨梅素(DMY)具有神经保护作用。本研究观察DMY对丙泊酚诱导的大鼠认知功能障碍的影响,并从小胶质细胞极化的角度探讨其机制。10周龄SD大鼠先进行腹腔注射丙泊酚(100 mg/kg)一次,然后灌胃给药DMY(每天200 mg/kg),共7天。Morris水迷宫测试发现DMY改善了丙泊酚诱导的认知功能障碍,Western blot分析发现DMY下调了丙泊酚诱导的大鼠海马组织中CD68和iNOS的表达,而上调海马组织中Arg1的表达。ELISA法检测发现DMY降低丙泊酚诱导的大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β) 和IL-6的水平,而增加IL-4和IL-10的水平(均P<0.05)。本研究结果阐明DMY改善了丙泊酚麻醉引起的大鼠认知功能障碍,其机制可能与调节小胶质细胞极化有关。 |
| 英文摘要: |
| Dihydromyricetin (DMY) has neuroprotective effect. The objective of this study is to observe the effect of dihydromyricetin (DMY) on cognitive impairment induced by propofol anesthesia in rats and explore its mechanism from microglia polarization. 10 weeks old male SD rats were administrated with propofol (100 mg/kg) by intraperitoneal injection once, then DMY (200 mg/kg per day) by gavage for 7 day. DMY improved the cognitive impairment induced by propofol through Morris water maze assay. Compared with the propofol group, expression of CD68 and iNOS in hippocampus was significantly down-regulated and expression of Arg1 in hippocampus was significantly up-regulated, the levels of serum TNF-α, IL-1β) and IL-6 was significantly decreased and levels of IL-4 and IL-10 were significantly increased in the freatment of propofol and DMY group (all P<0.05). This study elucidates that DMY improves cognitive impairment induced by propofol anesthesia in rats, which mechanism may be related with regulation of microglia polarization. |
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