景乐乐,王中群.基质小泡在糖尿病足截肢患者胫前动脉斑块内钙化中的作用分析.[J].中南医学科学杂志.,2018,(6):567-571.
基质小泡在糖尿病足截肢患者胫前动脉斑块内钙化中的作用分析
Analysis of the role of matrix vesicles in calcification of anterior tibial artery plaque in patients with diabetic foot amputation
投稿时间:2018-09-03  修订日期:2018-10-15
DOI:10.15972/j.cnki.43-1509/r.2018.06.002
中文关键词:  基质小泡  糖尿病足  截肢  斑块内钙化  动脉粥样硬化
英文关键词:matrix vesicles  diabetic Foot  amputation  plaque calcification  atherosclerosis
基金项目:
作者单位
景乐乐 江苏大学附属医院心内科,江苏 镇江 212001 
王中群 江苏大学附属医院心内科,江苏 镇江 212001 
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中文摘要:
      基质小泡是多种矿物形成细胞分泌的一种膜性结构。已有研究证实,基质小泡是无定型磷酸钙结晶成核的起始位点,但基质小泡在糖尿病足截肢患者胫前动脉斑块内钙化中的具体作用如何,现有的研究尚未阐明。本实验选取2015年10月~2018年1月在本院行糖尿病足截肢手术的患者共40例,根据组织钙定量测定将患者分为低钙化组(钙定量<4 mol/mg,n=20)及高钙化组(钙定量>4 mol/mg,n=20)。采用一系列形态学染色、分子生物学技术检测及统计学分析,结果显示:随着钙定量增加,基质小泡标记蛋白表达明显增加,高钙化组基质小泡水平高于低钙化组。Pearson 相关分析显示基质小泡水平与斑块内钙定量呈正相关(r=0.772 9,P<0.01)。ROC曲线显示曲线下面积为0.894(95% CI:0.788~0.999,P<0.01)。这些结果提示基质小泡水平与糖尿病动脉斑块内钙化的演进存在正相关,其水平对不同程度斑块内钙化有一定的预测价值。
英文摘要:
      Matrix vesicle is a membranous structure secreted by various mineral-forming cells. Studies have confirmed that matrix vesicles are the starting sites for the nucleation of amorphous calcium phosphate crystals. But the specific role of matrix vesicles in calcification of anterior tibial artery plaque in patients with diabetic foot amputation has not yet been fully elucidated. Forty patients with diabetic foot amputation were enrolled from October 2015 to January 2018. The patients were divided into a low calcification group (calcium quantification <4 mol/mg, n=20) and a high calcification group (calcium quantitation >4 mol/mg, n=20) according to calcium quantification. A series of morphological staining, molecular biology techniques and statistical analysis were performed. The results showed that the expression of matrix vesicle marker protein increased with the increase of calcium content. The level of matrix vesicles in the high calcification group was higher than that in the low calcification group. Pearson correlation analysis showed that the level of matrix vesicles was positively correlated with the calcium content in the anterior tibial artery plaque (r=0.772 9, P<0.01). ROC curve showed an area under the curve of 0.894 (95% CI:0.788-0.999, P<0.01). These results showed that the level of matrix vesicles is positively correlated with the progression of calcification in diabetic arterial plaques, and its level has certain predictive value for different degrees of plaque calcification.
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