| 耿春杰,黄俊会,李曾刚,尹志辉.FQ-PCR和ELISA法检测生殖器疱疹病毒抗原检测结果比较.[J].中南医学科学杂志.,2018,(5):526-528. |
| FQ-PCR和ELISA法检测生殖器疱疹病毒抗原检测结果比较 |
| Comparative research on detection results of genital herpes virus antigen by FQ-PCR and ELISA methods |
| 投稿时间：2018-05-09 修订日期：2018-06-01 |
| DOI：10.15972/j.cnki.43-1509/r.2018.05.021 |
| 中文关键词: 荧光定量PCR法 酶联免疫吸附测定法 疱疹病毒 抗原 敏感性 特异性 |
| 英文关键词:fluorescence quantitative PCR enzyme-linked immunosorbent assay herpes virus antigen sensitivity specificity |
| 基金项目: |
|
| 摘要点击次数: 534 |
| 全文下载次数: 555 |
| 中文摘要: |
| 本文对FQ-PCR和ELISA法检测生殖器疱疹病毒抗原的检测结果进行比较,为临床诊断治疗提供参考。分析邢台市第三医院皮肤性病科2015~2017年诊治的生殖器疱疹60例患者临床资料。采用酶联免疫吸附测定法(ELISA法)和荧光定量PCR法(FQ-PCR)检测样本中单纯疱疹病毒,检测结果不一致的样本采用单纯疱疹病毒通用引物分析。结果显示60例样本中FQ-PCR法检测阳性为46例,占76.7%,ELISA法检测阳性为48例,占80.0%,检测结果不一致样本为6例,占10.0%；1例FQ-PCR法阳性而ELISA法阴性样本经第二次检测后为阴性,3例ELISA法检测阳性而FQ-PCR法检测阴性样本经第二次检测后阳性为2例,阴性为1例；FQ-PCR法的敏感性、特异性、阳性预测值和阴性预测值分别为92.4%、96.3%、93.7%和94.5%,而ELISA法的上述指标分别为94.4%、96.3%、93.8%和95.8%。因此,ELISA法检测生殖器疱疹病毒抗原敏感性和特异性高,值得临床推广。 |
| 英文摘要: |
| The detection results of genital herpes virus antigen by FQ-PCR and ELISA were assayed.The clinical data of 60 cases of genital herpes diagnosed and treated by dermatology and Venerology in our hospital from 2015 to 2017 were analyzed. Herpes simplex virus was detected by enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative PCR (FQ-PCR). The samples with different results were analyzed by herpes simplex virus universal primers. The results showed that 46 cases were positive by FQ-PCR among 60 samples, accounting for 76.7%. At the same time, ELISA method showed that there were 48 positive cases, accounting for 80%.The in-consist results were 6 cases, accounting for 10%.The sensitivity, specificity, positive predictive value and negative predictive value of FQ-PCR and ELISA were 92.4%, 96.3%, 93.7%, 94.5%and 94.4%, 96.3%, 93.8%,95.8% respectively. So ELISA has high sensitivity and specificity to detect genital herpes virus antigen. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|