| 贺莎,严汉兴,阳学风,陈荣如,胡云,文美玲,左建宏.MicroRNA-4270在肝癌血浆和组织中的检测结果及分析.[J].中南医学科学杂志.,2018,(3):281-285. |
| MicroRNA-4270在肝癌血浆和组织中的检测结果及分析 |
| Test result of MicroRNA-4270 in hepatocellular carcinoma and its analysis |
| 投稿时间：2017-11-01 修订日期：2018-03-30 |
| DOI：10.15972/j.cnki.43-1509/r.2018.03.012 |
| 中文关键词: 肝癌 miR-4270 检测 临床分析 |
| 英文关键词:hepatocellular carcinoma microRNA-4270 detection clinical anlysis |
| 基金项目:国家自然科学基金 (81272960),湖南省科技厅重点项目资助 (2013WK2010,2014SK2015和2017SK2082),人社部重点项目〔2016〕176 号；天晴肝病研究基金(TQGB20140155)；衡阳市“科技创新创业人才团队”支持计划培育团队项目(衡人才发〔2017〕1号). |
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| 中文摘要: |
| MicroRNA作为新型肿瘤标志物被广泛研究报道,能提高肝癌早期的诊断率。本文主要利用实时荧光定量PCR(qRT-PCR)方法分别检测血浆及组织中miR-4270的表达水平,分析其表达差异及临床价值。结果表明在血浆及组织中,肝癌患者miR-4270的表达水平都明显高于正常人(P＜0.05),与患者的年龄、性别、乙肝病毒定量、肿块大小、临床分型均无显著相关性(P＞0.05)。通过ROC曲线,可以得出miR-4270在诊断肝癌时的敏感度和特异性。生存曲线分析表明肝癌中miR-4270高表达者生存时间低于低表达组(P＜0.05)。血浆和组织中肝癌的miR-4270表达量均上调,对肝癌患者的初步诊断及预后有一定意义。 |
| 英文摘要: |
| MicroRNAs as a kind of new tumor markers are widely reported,and it can improve the early diagnostic rate of liver cancer.In this passage,in order to analyze the expression and clinical value of microRNA-4270,Quantitative Real-time PCR(qRT-PCR) was used to examine the expression level of miR-4270 in the plasma samples and tissue specimens,respectively.The result indicated that,in the plasma and the tissue,the expression level of microRNA-4270 for liver cancer patients was significantly higher than normal people(P＜0.05). Nevertheless,it was not related to age,gender,viral hepatitis,tumor size and AFP(Alpha Fetal Protein)(P＞0.05).The ROC curve reflected the sensitivity and the specificity in liver cancer diagnosis. Kaplan-Meier showed that those patients may have shorter survival time with the higher expression level of microRNA-4270(P＜0.05).The expression of microRNA-4270 was upregulated in liver cancer,and it may have important significance in the diagnosis and prognosis for primary liver cancer . |
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