| 刘世敏,刘述成,李清.RNA干扰抑制细胞外基质COL6A1基因表达对肾癌786-0细胞增殖和侵袭的影响.[J].中南医学科学杂志.,2018,(3):276-280. |
| RNA干扰抑制细胞外基质COL6A1基因表达对肾癌786-0细胞增殖和侵袭的影响 |
| Targeting COL6A1 by RNA interference inhibits proliferation and invasion of renal urothelial carcinoma in 786-0 cells |
| 投稿时间：2017-11-06 修订日期：2018-02-25 |
| DOI：10.15972/j.cnki.43-1509/r.2018.03.011 |
| 中文关键词: 肾癌 COL6A1 增殖 侵袭 RNA干扰 |
| 英文关键词:renal carcinoma COL6A1 proliferation invasion RNA interference |
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| 中文摘要: |
| 研究小干扰RNA(siRNA)抑制COL6A1基因表达对肾癌786-0细胞增殖和侵袭能力的影响。设计合成靶向COL6A1基因的siRNA,瞬时转染肾癌细胞株786-0,利用实时定量聚合酶链反应(qRT-PCR)和Western blot检测RNA干扰后COL6A1基因沉默效果,在RNA干扰后1、2、3天应用噻唑蓝(MTT)比色法检测细胞增殖；应用transwell小室侵袭实验检测肾癌细胞侵袭能力。靶向COL6A1的siRNA成功抑制肾癌细胞株786-0中COL6A1信使RNA(mRNA)及蛋白表达(P<0.05),经MTT测定,肾癌细胞生长受到抑制(P<0.05)；transwell小室侵袭实验表明,COL6A1被抑制后,肾癌穿膜细胞数明显减少,侵袭力降低(P<0.05)。应用siRNA技术可有效地抑制COL6A1基因的表达,抑制786-0细胞的体外增殖和侵袭。 |
| 英文摘要: |
| The aim of this study was to investigate effects of COL6A1 down-regulation by RNA interference (RNAi) on proliferation and invasion of renal carcinoma cell line 786-0. SiRNAs targeting COL6A1 gene were designed, and transiently transfected into renal carcinoma cell line 786-0. The transcription level of COL6A1 was detected by RT-PCR. Activities of COL6A1 protein were measured by Western blotting. The cell proliferation was detected by MTT at day 1, day 2, and day 3 after transfection. The cell’s invasion ability was performed on Matrigel transwell assay. The results showed that the levels of COL6A1 mRNA and protein was significantly reduced after RNAi(P<0.05). MTT assay showed that the proliferation ability of 786-0 cell was decreased due to RNA interference(P<0.05). Transwell assay showed that invasion ability of 786-0 cell was reduced obviously due to COL6A1 RNAi(P<0.05). The ability of proliferation and invasion in 786-0 cells can be inhibited by RNAi-targeting COL6A1. |
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