| 许继志,陈铁强,张路.PI3k/AKT信号通路在宫内发育迟缓新生大鼠海马组织中的表达及意义.[J].中南医学科学杂志.,2018,(2):127-131. |
| PI3k/AKT信号通路在宫内发育迟缓新生大鼠海马组织中的表达及意义 |
| The role of PI3K/AKT signal pathway in rat’s hippocampus withintrauterine growth restriction |
| 投稿时间：2017-10-09 修订日期：2017-11-29 |
| DOI：10.15972/j.cnki.43-1509/r.2018.02.004 |
| 中文关键词: 宫内发育迟缓 海马 PI3k/AKT信号通路 神经发育 |
| 英文关键词:intrauterine growth restriction hippocampus PI3K/AKT signal pathway neuronal development |
| 基金项目:湖南省卫生计生委科研计划课题:肌内效贴治疗痉挛型脑瘫合并膝患儿的疗效观察(B2016228). |
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| 中文摘要: |
| 宫内发育迟缓(IUGR)患儿可出现认知能力异常等神经系统后遗症,PI3K/AKT信号通路可能参与此过程。本文主要探讨PI3k/AKT信号通路与IUGR新生儿神经发育障碍发病机制的关系。以孕期低蛋白饮食法建立IUGR新生大鼠模型,并检测海马中PI3K,AKT等mRNA和蛋白在两组中的表达差异。在大鼠出生后24 h内,IUGR组的仔鼠大脑质量明显降低(P<0.05)；IUGR组海马的AKT和PI3K mRNA和蛋白的表达水平明显升高(P<0.01)。在出生7天时,IUGR组海马的 AKT和PI3K mRNA较对照组也明显升高。根据上述结果得出PI3k/AKT信号通路可能参与了IUGR神经发育异常的发病过程,提示PI3k/AKT信号通路在IUGR神经发育中起着重要的作用。 |
| 英文摘要: |
| Many studies testified that fetuses with IUGR are at increased risk for cognitive differences and other Neuropsychological disorders during childhood.Previous studies have demonstrated that PI3K/AKT signal pathway plays an important role in IUGR.The primary goal of our study was to identify the hippocampal abnormal expression profiles of PI3K/AKT signal pathway to give a better understanding on IUGR neurological pathogenesis.The model of IUGR rats was established by feeding low protein diet during pregnancy.RT-PCR and western blot were used to identify different expression of PI3k,AKT in the hippocampi of rats with IUGR.On the 24h after birth,the hippocampus of the rats in the IUGR group was smaller than that of the rats in the control group.On the day of 24h,AKT and PI3K mRNA expression levels were significantly increased in the IUGR group (all P<0.05),the results of the Western blot indicated that IUGR significantly increased hippocampal PI3K,AKT expression.On the day of 7 after birth,AKT and PI3K mRNA expression levels were significantly increased in the IUGR group (all P<0.05).IUGR can affect hippocampal PI3K/AKT signal pathway characteristic;it hypothesized that PI3K/AKT signal pathway may play important roles in the regulation of neuronal development in the IUGR. |
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