| 王文松,黄英,李清叶,刘芳,张杨,肖国华,谭晖.利用组织芯片分析DJ-1蛋白在非霍奇淋巴瘤组织中的定位表达及意义.[J].中南医学科学杂志.,2018,(2):119-122, 135. |
| 利用组织芯片分析DJ-1蛋白在非霍奇淋巴瘤组织中的定位表达及意义 |
| Analysis of the localization, expression and significance of DJ-1 proteinin non-Hodgkin lymphoma tissue by tissue chip |
| 投稿时间：2017-12-07 修订日期：2018-02-25 |
| DOI：10.15972/j.cnki.43-1509/r.2018.02.002 |
| 中文关键词: 非霍奇金淋巴瘤 组织芯片 DJ-1蛋白表达 |
| 英文关键词:non-Hodgkin lymphoma tissue chip DJ-1 protein expression |
| 基金项目:国家自然科学基金项目(81100375)；湖南省教育厅课题(14C0140)；湖南省研究生创新项目(课题编号: CX2017B553)；衡阳市科技局项目(2015KJ20). |
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| 中文摘要: |
| DJ-1是PARK7基因编码的一种蛋白质,其过表达与许多恶性肿瘤的发生、侵袭、转移及预后密切相关。然而,有关DJ-1蛋白在非霍奇金淋巴瘤(NHL)中的表达与NHL生物学特性的关系尚未见文献报导。本文检测了61例NHL组织中DJ-1蛋白在细胞核内外的表达情况,发现DJ-1蛋白在细胞浆内高表达的情况较细胞核内更为显著( 93.4%、72.1%),且都显著高于非肿瘤组织(28.6%、14.3%)。此外,DJ-1蛋白的高表达率与NHL的恶性程度正相关,DJ-1蛋白在惰性、侵袭性和高侵袭性NHL中核高表达率为52.2%、82.9%和100%；浆高表达率为82.6%、100%和100%。结果提示DJ-1蛋白在NHL细胞内的定位可能是一个动态过程,而这与肿瘤的发生和发展密切相关；DJ-1与NHL的转移和侵袭等恶性进程密切相关,DJ-1是极具潜力的NHL相关的肿瘤生物学标记物。 |
| 英文摘要: |
| DJ-1/PARK7 is a gene with oncogenic potential, and its overexpression is strongly linked with the occurrence, invasion, metastasis and prognosis of numerous malignant tumors. However, the relationship between DJ-1 protein expression and the biological characteristics of non-Hodgkinlymphoma (NHL) was not previously reported in the literature. This research investigated the intracellular distribution of DJ-1 protein in NHL tissues obtained from 61 patients, finding that DJ-1 proteins located in the cytoplasm at a higher compression ratio than those in the nucleus (93.4%, 72.1%, respectively), and both were remarkably higher than those in non-tumor tissues (28.6%, 14.3%,respectively). In addition, tissue expression of DJ-1 protein is positive to the malignancy of NHL:for indolent, aggressive and highly aggressive NHL,DJ-1 protein expressing rates in the cytoplasm were 52.2%, 82.9% and 100%, and in the nucleus were 82.6%、100%和100%, respectively.The results indicate that the localization of DJ-1 protein in NHL cells may be a dynamic process, which is closely associated with development and progression of lymphoma; DJ-1 is closely related to malignant processes such as metastasis and invasion of NHL; DJ-1 is a promising NHL-related oncology biomarker. |
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