| 唐义信,刘厂辉,占凡,罗孝天,刘超研,张国刚.血管过氧化物酶1在Ang-Ⅱ诱导血管平滑肌细胞表型转化中的作用及机制.[J].中南医学科学杂志.,2018,(1):61-66. |
| 血管过氧化物酶1在Ang-Ⅱ诱导血管平滑肌细胞表型转化中的作用及机制 |
| The role and mechanism of vascular peroxidase 1 in Ang-Ⅱ-induced vascular smooth muscle cell phenotype transformation |
| 投稿时间：2017-09-06 修订日期：2017-12-20 |
| DOI：10.15972/j.cnki.43-1509/r.2018.01.014 |
| 中文关键词: 血管平滑肌细胞表型转化 氧化应激 血管过氧化物酶1 血管紧张素Ⅱ |
| 英文关键词:Vascular smooth muscle cells synthetic phenotype oxidative stress VPO1 Ang-Ⅱ |
| 基金项目:国家自然科学基金(81170216),湖南省重点科技专项(2016SK1001-3). |
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| 中文摘要: |
| 血管平滑肌细胞(VSMCs)表型转化在动脉粥样硬化等血管增生性疾病中起重要作用,但具体调节机制仍不明确。为探讨血管过氧化物酶1(VPO1)在血管紧张素Ⅱ(Ang-Ⅱ)诱导VSMCs表型转化中的作用及机制,予以Ang-Ⅱ培养VSMCs,结果发现Ang-Ⅱ升高VSMCs内VPO1的表达,伴随着SM22α、α-SMA表达下降和OPN、KLF4表达升高。VPO1基因沉默后可明显抑制Ang-Ⅱ诱导的SM22α、α-SMA表达下降和OPN、KLF4表达升高,同时抑制HOCl的生成。HOCl培养VSMCs使SM22α、α-SMA表达下降和OPN、KLF4表达升高。研究结果表明VPO1通过调节KLF4的表达在Ang-Ⅱ诱导VSMCs表型转化中起重要作用。 |
| 英文摘要: |
| Vascular smooth muscle cells(VSMCs) phenotype transformation plays an important role in various vascular proliferative diseases such as atherosclerosis,while the regulation mechanism of VSMCs phenotype transformation remains unclear.To explore the role and mechanism of vascular peroxidase 1(VPO1) in Ang-Ⅱ induced VSMCs phenotype transformation,VSMCs were incubated with Ang-Ⅱand it found that Ang-Ⅱ increased the expression of VPO1 in VSMCs,accompanied by decreased expression of SM22α,α-SMA and elevated expression of OPN and KLF4.The silencing of VPO1 could obviously inhibit the decrease of SM22α and α-SMA induced by Ang-Ⅱ,the increase of OPN and KLF4 expression while the inhibition of HOCl production.HOCl cultured VSMCs that decreased the expression of SM22α,α-SMA and elevated expression of OPN and KLF4.The results showed that VPO1 plays a significant role in Ang-Ⅱ induced VSMCs phenotype transformation through regulating KLF4 protein expression. |
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