| 娄峥,周雅倩,徐献,雷文枚,任贤.丹参乙酸镁减轻脑缺血/再灌注诱导的神经细胞凋亡作用及机制.[J].中南医学科学杂志.,2017,(5):465-470. |
| 丹参乙酸镁减轻脑缺血/再灌注诱导的神经细胞凋亡作用及机制 |
| Effect of magnesium lithospermate B on cerebral ischemia/reperfusion- induced apoptosis and the underlying mechanisms |
| 投稿时间：2016-12-01 修订日期：2017-07-26 |
| DOI：10.15972/j.cnki.43-1509/r.2017.05.009 |
| 中文关键词: 丹参乙酸镁 缺血/再灌注 氧化应激 |
| 英文关键词:magnesium lithospermate B ischemia/reperfusion injury oxidative stress |
| 基金项目:湖南省教育厅一般项目(15C0161)药学类专业校企合作人才培养示范基地(湘教通[2014]272号). |
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| 中文摘要: |
| 目的 探讨丹参乙酸镁对于大鼠脑缺血/再灌注损伤中的保护作用及其机制。方法动物实验采用大鼠线栓法缺血/再灌注模型,使大鼠脑缺血2 h后,再灌注24 h。细胞实验采用NG108-15神经细胞株缺氧/复氧模型,低氧无糖培养2 h后,复氧24 h。检测神经细胞凋亡、NADPH氧化酶 (NOX)活性及H2O2水平。结果与模型组比较,丹参乙酸镁组大鼠脑组织神经细胞凋亡明显下降,NOX活性和H2O2水平均降低。结论丹参乙酸镁具有抗脑缺血/再灌注损伤的作用,其机制与抑制NOX活性,减少H2O2生成有关。 |
| 英文摘要: |
| Objective To investigate whether lithospermate B is able to protect the rat brain from ischemia/reperfusion injury and the underlying mechanism.MethodRats were subjected to 2 h of cerebral ischemia and 24 h of reperfusion to establish anischemia/reperfusion injury model.In a NG108-15 nerve cell hypoxia/reoxygenation (H/R) injury model,cells were cultured in 2 h of hypoxia and 24 h of reoxygenation.And cellular apoptosis,nicotinamide adenine dinucleotide phosphate-oxidase (NOX)activity,and H2O2 content were examined.ResultAdministration of salvia magnesium lithospermate B reduced apoptosis of nerve cells with a decrease in nicotinamide adenine dinucleotide phosphate-oxidase activity and H2O2 production in the rat brains,compared with model group.In the experiments,the number of iHoechst staining positive cells,nicotinamide adenine dinucleotide phosphate-oxidase activity and H2O2 level were decreased by the intervention of drugs.ConclusionThe results suggest that lithospermate B is able to protect the brain from ischemia/reperfusion oxidative injury,which is related to the inhibition of nicotinamide adenine dinucleotide phosphate-oxidase and a reduction of reactive oxygen species production. |
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