| 李柱,农巧红,童刚领,王树滨.咯萘啶逆转人乳腺癌MCF-7/ADM细胞耐药性及机制探讨.[J].中南医学科学杂志.,2017,(4):346-349. |
| 咯萘啶逆转人乳腺癌MCF-7/ADM细胞耐药性及机制探讨 |
| Reversal effect and mechanism of pyronaridine on drugresistance of breast cancer cell MCF-7/ADM |
| 投稿时间：2017-02-20 修订日期：2017-05-23 |
| DOI：10.15972/j.cnki.43-1509/r.2017.04.005 |
| 中文关键词: 咯萘啶 阿霉素 乳腺癌 MCF-7/ADM细胞 逆转耐药 细胞凋亡 |
| 英文关键词:pyronaridine adriamycin breast cancer MCF-7/ADM cell reversing drug-resistance apoptosis |
| 基金项目:深圳市创新知识计划基础研究项目(JCYJ2015040309144300). |
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| 中文摘要: |
| 目的 在明确咯萘啶(PND)逆转人乳腺癌细胞MCF-7/ADM耐药性的药效基础上,初步探索作用机制。方法采用MTT法检测单用阿霉素(ADM)和联用咯萘啶(PND)对人乳腺癌敏感细胞MCF-7和耐药细胞MCF-7/ADM的抑制作用,得出半数抑制浓度(IC50),并计算耐药倍数和逆转倍数。Western blot检测细胞中Fas和Caspases-3蛋白表达。结果ADM对MCF-7和MCF-7/ADM的IC50分别为1.399 μg/mL和43.885 μg/mL,耐药倍数为31.4倍。PND(0.5 μg/mL)联合ADM作用MCF-7/ADM的IC50为3.246 μg/mL,逆转倍数为13.5倍,并能提高耐药MCF-7/ADM中Fas和Caspases-3蛋白表达。结论PND能够逆转人乳腺癌细胞MCF-7/ADM耐药性,通过上调膜蛋白Fas表达,增加MCF-7/ADM对ADM的敏感性,从而促进细胞凋亡。 |
| 英文摘要: |
| Objective Based on the effect of pyronaridine (PND) in reversing drug- resistance of human breast cancer cell MCF-7/ADM,to explore the mechanism of action.MethodsThe inhibitory effect of simple adriamycin (ADM) and combined with PND on sensitive human breast cancer cell MCF-7 and drug-resistance MCF-7/ADM was detected by MTT assay,and to calculate median inhibitory concentration,resistance index and reversal index.Cells were designated as MCF-7/ADM group,MCF-7/ADM+ADM group and MCF-7/ADM+ADM+PND group.Western blot detected the expression of Fas and Caspases-3 in cells.ResultsThe IC50 of ADM to MCF-7 and MCF-7/+ADM were 1.399 μg/mL和43.885 μg/mL,respectively,and the resistance index was 31.4.The IC50 of PND combined with ADM to MCF-7/ADM was 3.246 μg/mL,and the reversal index was 13.5.Compared with MCF-7/ADM group,the expression of Fas and Caspases had no statistic difference (P>0.05) in MCF-7/ADM +ADM group,but the expression increased significantly in MCF-7/ADM +ADM+PND group (P<0.05).ConclusionPND could reverse drug- resistance of MCF-7/ADM by up-regulating expression of membrane protein Fas,which increased sensibility of MCF-7/ADM to ADM,and induced the cell apoptosis. |
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