| 陈斌,符勇,夏雪,郭伟明,金浩成,王晓旭.MicroRNA-638在骨肉瘤中表达下调及对其细胞增殖的影响.[J].中南医学科学杂志.,2017,(4):341-345, 363. |
| MicroRNA-638在骨肉瘤中表达下调及对其细胞增殖的影响 |
| Effect of MicroRNA-638 expression on the proliferation ofhuman osteosarcoma cells |
| 投稿时间：2017-02-10 修订日期：2017-06-18 |
| DOI：10.15972/j.cnki.43-1509/r.2017.04.004 |
| 中文关键词: 骨肉瘤 MG63 U2OS MicroRNA-638 细胞增殖 |
| 英文关键词:osteosarcoma MG63 U2OS MicroRNA-638 cell proliferation |
| 基金项目:湖南省教育厅资助项目(15C1215). |
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| 中文摘要: |
| 目的 探讨miR-638在人骨肉瘤(OS)细胞增殖行为中的影响。方法通过实时定量聚合酶链反应(qRT-PCR)检测miRNA-638在人OS组织及癌旁组织、人OS细胞株(MG63、U2OS、HOS、SaOS2)和正常人成骨NHOst细胞株中的表达；用LipofectamineTM 2000转染miR-638模拟物或抑制物,细胞增殖检测试剂盒(CCK-8)检测转染后的吸光光度值,平板克隆形成实验检测菌落数量。结果miR-638在各组细胞中均有表达；OS细胞株中miR-638的表达明显下调(P＜0.05)；高表达miR-638能抑制OS MG63和U2OS细胞的增殖(P＜0.05)。 结论miR-638高表达能抑制OS细胞的增殖。 |
| 英文摘要: |
| Objective To investigate the role of miR-638 in biological behavior of osteosarcoma cell.MethodsThe expression of miR-638 in osteosarcoma MG63 and U2OS cells was detected by RT-PCR.miRNA-638 mimics (inhibhors) were transfected into MG63 and U2OS by using LipofectamineTM2000 to upregulate(deregulate) the expression of miRNA-638.The absorbance value in 24 h,48 h,96 h and 72 h following the transfection was detected by CCK-8.And colony forming assay was used to detect colony counts over 30 cells after 2 weeks.ResultsMiR-638 expressed in each group.The expression of miR-638 was down regulated (P<0.001) in the 36 groups (56.25%) compared with the corresponding non cancerous tissues.Mir-638 expression can significantly reduce the MG63 and U2OS cells growth rate (P<0.05),on the contrary,the suppression of miR-638 expression significantly promoted the growth of cells of U2OS and MG63 (P<0.05).ConclusionsHigh expression of miR-638 significantly inhibits the proliferation of osteosarcoma cells.MiR-638 may become a new target for the early diagnosis and treatment of osteosarcoma. |
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