| 吕秀,雷明盛,贾凤玲,李赛,张楚红,胡甜,贺莎,严汉兴,胡云,陈荣如,谭贻兮,文美玲,左建宏.循环miR-4731-3p在肺癌患者血浆中的表达及临床意义.[J].中南医学科学杂志.,2017,(3):255-258. |
| 循环miR-4731-3p在肺癌患者血浆中的表达及临床意义 |
| Expression and clinical significance of miR-4731-3p inplasma of patients with lung cancer |
| 投稿时间：2016-10-25 修订日期：2016-12-28 |
| DOI：10.15972/j.cnki.43-1509/r.2017.03.010 |
| 中文关键词: 肺癌 微小RNA-4731-3p 诊断 预后 |
| 英文关键词:lung cancer MiR-4731-3p diagnosis prognosis |
| 基金项目:国家自然科学基金(81272960),湖南省科技厅项目(2013WK2010),人社部重点项目[2016]176号,省卫计委项目[B2014-171],张家界市科技局项目[2015GK1128]. |
| 作者 | 单位 | | 吕秀 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001
南华大学医学院肿瘤研究所 | | 雷明盛 | 张家界市人民医院呼吸内科 | | 贾凤玲 | 南华大学医学院肿瘤研究所 | | 李赛 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 张楚红 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 胡甜 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 贺莎 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 严汉兴 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 胡云 | 南华大学医学院肿瘤研究所 | | 陈荣如 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 谭贻兮 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 文美玲 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001 | | 左建宏 | 南华大学附属南华医院肿瘤内科,湖南 衡阳 421001
南华大学医学院肿瘤研究所 |
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| 中文摘要: |
| 目的 探讨microRNA-4731-3p(miR-4731-3p)在肺癌血浆中的表达及其临床意义。方法采用实时荧光定量PCR(qRT-PCR)方法检测40例肺癌患者和40例健康人(作为对照)血浆miR-4731-3p的表达水平,分析其与临床病理参数和预后的相关性。绘制受试者工作特征曲线(ROC),评价血浆miR-4731-3p表达水平对原发性肺癌的诊断价值。结果肺癌血浆中miR-4731-3p的表达水平(0.11±0.05)显著高于健康人(0.05±0.03)(P＜0.001)；miR-4731-3p表达水平与癌胚抗原(CEA)正相关(P＜0.05),与患者性别、年龄、吸烟、病理类型、肿瘤大小、临床分期、远处转移均无明显相关性(P＞0.05)。血浆miR-4731-3p在ROC曲线下面积(AUC)为0.835(95% CI =0.750~0.921)。当miR-4731-3p临界值取0.048时,对肺癌诊断的灵敏度为0.950,特异度为0.575。Kaplan-Meier分析显示肺癌血浆中miR-4731-3p低表达者生存时间明显高于高表达组(P＜0.05)。结论血浆miR-4731-3p在肺癌血浆中表达上调,可作为肺癌诊断及预后判断的潜在分子标志物。` |
| 英文摘要: |
| Objective To investigate the expression of microRNA-4731-3p (miR-4731-3p) in the plasma of patients with lung cancer and to determine the correlation between miR-4731-3p level and clinical parameters.MethodsQuantitative real-time PCR (qRT-PCR) was used to examine the plasma level of miR-4731-3p in 40 patients with lung cancer and 40 healthy persons (control).The correlation between the plasma miR-4731-3p level and clinicopathological features and prognosis were investigated.Receiver operating characteristic (ROC) curve were drawn to evaluate the diagnosis value of miR-4731-3p expression in plasma for lung cancer.ResultsThe plasma miR-4731-3p was significantly up-regulated in patients with lung cancer as compared with healthy subjects.The expression of miR-4731-3p was associated with carcino-embryonic antigen (CEA) (P＜0.05),while it was not associated with gender,age,smoking,pathology type,tumor size,clinical stage and distant metastasis (P＞0.05).The area under the ROC curve (AUC) according to the miR-4731-3p expression in plasma was 0.835(95% CI =0.750~0.921),when the critical value was 0.048.The sensitivity for lung cancer diagnosis and specific degrees were 0.950 and 0.575 respectively.The expression of miR-4731-3p was correlated with overall survival of lung cancer (P＜0.05).ConclusionThe expression of miR-4731-3p is up-regulated in tumor,and it may be a potential biomarker for the diagnosis and prognosis evaluation of lung cancer. |
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