| 全文婕,史晓欣,熊润德,张开,喻翠云,贺冬秀.野菊花抗肿瘤活性部位筛选研究.[J].中南医学科学杂志.,2017,(3):242-246. |
| 野菊花抗肿瘤活性部位筛选研究 |
| Screening of antitumor active fractions of Chrysanthemum Indicum |
| 投稿时间：2016-12-31 修订日期：2017-03-23 |
| DOI：10.15972/j.cnki.43-1509/r.2017.03.007 |
| 中文关键词: 野菊花 肿瘤细胞 抗肿瘤活性 抗肿瘤活性部位 |
| 英文关键词:Chrysanthemum indicum tumor cells antitumor activity screening of antitumor activity fractions |
| 基金项目:湖南省中医药科研计划重点项目(201412)；湖南省教育厅科学研究项目(16K079). |
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| 中文摘要: |
| 目的 筛选野菊花的抗肿瘤活性部位并初步探讨其机制。方法利用形态观察、CCK-8、Hoechst染色和划痕实验评价野菊花总提取物(CIE)及其石油醚部位(PEE)、乙酸乙酯部位(EAE)、正丁醇部位提取物(NAE)对人肺癌细胞A549、人慢性骨髓性白血病细胞K562、人乳腺癌细胞MCF-7和人肝癌细胞HepG2的抗肿瘤活性。结果CIE及PEE、EAE、NAE>150 μg/mL对A549、K562和MCF-7细胞形态影响明显且CIE及PEE、EAE、NAE(150~250 μg/mL)对上述三种细胞增殖抑制作用呈量效相关性,PEE对MCF-7细胞抑制效果最显著；EAE对MCF-7细胞的诱导凋亡作用和迁移抑制作用相对较好。结论野菊花具有抗肿瘤活性,抗肿瘤活性较强的部位为石油醚和乙酸乙酯部位,其抗肿瘤机制可能与抑制细胞增殖、迁移和诱导细胞凋亡有关,这为进一步研究开发野菊花提供基础。 |
| 英文摘要: |
| Objective To evaluate the antitumor activity and screen the antitumor activity fractions of Chrysanthemum Indicum.MethodsMorphological observation,CCK-8,Hoechst nuclei staining and wound healing assay were used to evaluate the antitumor activity of CIE,PEE,EAE and NAE in tumor cells including human lung cancer cells (A549), human erythroleukemia cells (K562), human breast cancer cells (MCF-7), and human liver cancer cells (HepG2).ResultsShrinkage and fragmentation of cells could be seen obviously under the fluorescent inverted microscope when A549, K562 and MCF-7 cells were treated with CIE,PEE,EAE,NAE when their concentrations were above 150μg/mL. The proliferation of A549,K562 and MCF-7 cells can be inhibited by CIE,PEE,EAE,NAE in a dose-dependent manner in the concentration from 150μg/mL to 250μg/mL, PEE showed the best inhibition effects in MCF-7. EAE showed the best apoptosis-inducing effect in MCF-7, and did the best in resisting MCF-7 cell migration .ConclusionChrysanthemum indicum has antitumor activity and its mechanism might be associated with induction of apoptosis and inhibition of migration; the antitumor activity sites were the petroleum ether and ethyl acetate fractions, which provides the basis for further study in single compounds isolation. |
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