| 谢娟,唐霞,李国庆,刘艳萍,李熠,冯聚玲,许丽芳,王超.丝裂霉素对胃癌细胞SGC-7901miRNA表达谱的影响.[J].中南医学科学杂志.,2017,(2):127-131. |
| 丝裂霉素对胃癌细胞SGC-7901miRNA表达谱的影响 |
| MicroRNA profiling of gastric cancer SGC-7901 after mitomycin C treatment |
| 投稿时间：2016-10-28 修订日期：2016-02-10 |
| DOI：10.15972/j.cnki.43-1509/r.2017.02.005 |
| 中文关键词: 丝裂霉素 MiRNA表达谱 胃癌 SGC-7901 |
| 英文关键词:mitomycin miRNA expression profile gastric cancer SGC-7901 |
| 基金项目:湖南省教育厅科学研究项目(16C1401)资助,湖南省教育厅优秀青年基金项目(13B098)资助. |
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| 中文摘要: |
| 目的 分析丝裂霉素(MMC)治疗对胃癌细胞miRNA表达谱的影响,为MMC的胃癌治疗机制提供理论参考。方法 体外培养胃癌SGC-7901细胞,根据噻唑兰(MTT)法评估MMC的作用效果和作用浓度；利用miRNA芯片技术评估MMC干预后SGC-7901的miRNA表达谱改变,并通过qRT-PCR验证差异表达miRNA。 结果 SGC-7901细胞生长抑制率与MMC作用浓度间存在正相关；MMC干预48 h后SGC-7901细胞有59个miRNA出现表达改变,与正常胃黏膜上皮细胞GES-1相比,SGC-7901胃癌细胞的miR-205*和miR-3924等11个miRNAs表达上调,而miR-498,miR-18b,miR-196b,miR-1247等22个miRNA表达下调；经MMC干预后,部分异常表达得到恢复。 结论 MMC可能调控miR-498在内的59个miRNA在胃癌细胞的表达。 |
| 英文摘要: |
| Objective To evaluate the mitomycin C (MMC) treatment on the miRNA expression profile in gastric cancer cells,and provide the theoretical evidences for MMC treatment on gastric cancer.Methods The effects and optimum concentration of MMC were evaluated in the cultured SGC-7901 cells by MMT. The miRNA microarray technique was used for the exploration of miRNA profile of SGC-7901 cells after MMC treatment and the different expression of candidate miRNA was further confirmed by qRT-PCR.Results The inhibition rate of SGC-7901 cells was negatively correlated with the MMC concentration. The 60 miRNAs have changed expression in SGC-7901 cells 48 hours after MMC treatment. Among them,11 miRNAs included miR-498 and miR-1247 were up-regulated,and miR-205* and miR-3924 were down-regulated. Some abnormal expression changes recovered after MMC treatment. Conclusion MMC can regulate the expression of 60 miRNAs including miR-498 in gastric cancer cells. |
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