| 莫中成,彭凤玲,张梅英,欧含笑,谢远杰,陈赛男,石金凤,龙双涟.血管紧张素Ⅱ2型受体活化促进缺氧/复氧损伤PC12细胞的生长.[J].中南医学科学杂志.,2017,(1):35-41. |
| 血管紧张素Ⅱ2型受体活化促进缺氧/复氧损伤PC12细胞的生长 |
| Angiotensin II type 2 receptor activation promotes the growth ofPC12 cells induced by hypoxia/reoxygenation |
| 投稿时间：2017-06-23 修订日期：2017-10-28 |
| DOI：10.15972/j.cnki.43-1509/r.2017.01.009 |
| 中文关键词: 血管紧张素Ⅱ2型受体 PC12细胞 缺氧/复氧损伤 凋亡 |
| 英文关键词:angiotensin II type 2 receptor PC12 cells hypoxia/reoxygenation injury apoptosis |
| 基金项目:湖南省自然科学基金(2017JJ6133)；南华大学大学生研究性学习和创新性实验计划项目(2017NH053XJXZ). |
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| 中文摘要: |
| 目的 探讨血管紧张素Ⅱ2型受体(AT2R)活化对缺氧/复氧(H/R)损伤PC12细胞生长的影响。方法PC12细胞是大鼠肾上腺嗜铬细胞瘤细胞株,具有神经内分泌细胞的一般特征。将PC12细胞用连二亚硫酸钠(Na2S2O4)处理后复制H/R损伤的神经细胞模型；H/R损伤PC12细胞分别予以AT2R拮抗剂(PD123319)、AT2R激动剂(CGP42112)进行处理,采用MTT法观察细胞存活率的变化；以逆转录—聚合酶链式反应(RT-PCR)检测Bax、Bcl-2 mRNA表达的变化。结果PC12细胞经40mmol/LNa2S2O4处理1 h后,MTT法检测的细胞存活率约为55~60%,提示复制H/R损伤模型成功；H/R损伤PC12细胞经CGP42112处理后,细胞存活率显著增高,而Bax mRNA 表达下调,Bcl-2 mRNA 表达上调；而PD123319处理后,细胞存活率显著下降, Bax mRNA 表达上调,Bcl-2 mRNA 表达则下调。结论AT2R活化可改善H/R损伤的PC12细胞的生长,其机理可能与其上调Bcl-2/Bax的比值有关。 |
| 英文摘要: |
| Objective To investigate the effects of activated angiotensin II type 2 receptor (AT2R) on the growth of PC12 cells induced by hypoxia/reoxygenation (H/R).MethodsPC12 cells were treated with Na2S2O4 for 1 h to establish the H/R model.Then the cells were treated with AT2R antagonist (PD123319) or AT2R agonist (CGP42112),respectively.The survival rate of cells was observed by MTT,the expression of Bax/Bcl-2 mRNA was detected through reverse transcription PCR.ResultsThe survival ratio of cells is about 55~60% in the cells treated with 40mmol/L Na2S2O4 and shows that the H/R injury model is successfully established in the PC12 cells.Compared with the control groups,the survival rate of cells is increased,Bax mRNA expression is down-regulated and Bcl-2 mRNA expression is up-regulated in the H/R injury PC12 cells treated with CGP42112.While in the H/R injury PC12 cells treated with PD123319,the survival rate of cells is decreased,Bax mRNA expression is up-regulated and Bcl-2 mRNA expression is down-regulated.ConclusionAT2R activation can improve the growth of PC12 cells damaged by H/R,which the mechanism is related with the upregulation of Bcl-2/Bax ratio. |
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