| 杨晓燕,向琼,殷杰,虞佳,甘润良,雷小勇.MiR-195对BEL-7402/5-FU细胞5-氟尿嘧啶耐药性的影响.[J].中南医学科学杂志.,2017,(1):32-34, 47. |
| MiR-195对BEL-7402/5-FU细胞5-氟尿嘧啶耐药性的影响 |
| Effects of MicroRNA-195 on 5-FU Resistance in BEL-7402/5-FU Cells |
| 投稿时间：2017-04-10 修订日期：2017-09-21 |
| DOI：10.15972/j.cnki.43-1509/r.2017.01.008 |
| 中文关键词: MiR-195 BEL-7402/5-FU细胞 5-氟尿嘧啶 耐药性 |
| 英文关键词:MiRNA-195 BEL-7402/5-FU cells 5-FU drug resistance |
| 基金项目:国家自然科学基金资助(81372579);湖南省卫生厅医药卫生资助科研项目(B2014-048);湖南省教育厅一般课题(13C832). |
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| 中文摘要: |
| 目的 探讨miR-195对BEL-7402/5-FU细胞5-氟尿嘧啶药物敏感性的影响。方法采用qRT-PCR检测BEL-7402细胞与BEL-7402/5-FU细胞中miR-195的表达水平。将miR-195质粒载体瞬时转染至BEL-7402/5-FU细胞；MTT检测细胞药物敏感性的改变。结果相比于BEL-7402细胞,miR-195在BEL-7402/5-FU细胞中表达水平下调；miR-195转染组的细胞增殖抑制率较miRNA阴性对照组及未转染组明显增高。结论MiR-195能够增加BEL-7402/5-FU细胞对5-氟尿嘧啶的药物敏感性,抑制细胞增殖。 |
| 英文摘要: |
| Objective This study investigated the effect of miR-195 on 5-FU resistance in BEL-7402/5-FU Cells.MethodsqRT-PCR analyzed expression of miR-195 between BEL-7402 and BEL-7402/5-FU cells.MiR-195 was transiently transfected into BEL-7402/5-FU cells.Drug sensitivity of the cells to 5-FU was analyzed by MTT.ResultsThe expression of miR-195 was downregulated in BEL-7402/5-FU cells compared to parental BEL-7402 cells.MTT results showed that miR-195 transfected cells had a higher cell inhibition rate than untreated cells or negative control.ConclusionsMiR-195 could sensitize BEL-7402/5-FU cells to 5-FU and inhibited cell proliferation. |
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