| 全小青,李武,曾怀才.PFOS致SH-SY5Y细胞凋亡及可能机制.[J].中南医学科学杂志.,2016,(4):395-399. |
| PFOS致SH-SY5Y细胞凋亡及可能机制 |
| The Study of SH-SY5Y Cells Apoptosis Induced by PFOS and Its Mechanism |
| 投稿时间：2016-03-03 修订日期：2016-06-28 |
| DOI： |
| 中文关键词: 全氟辛烷磺酸钾盐 成人神经母瘤细胞 氧化应激 凋亡 |
| 英文关键词:PFOS SH-SY5Y cells oxidative stress apoptosis |
| 基金项目:国家自然科学基金(81273026),湖南省卫生厅(B2011-044),衡阳市科技局(2011KS9). |
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| 中文摘要: |
| 目的 探讨全氟辛烷磺酸盐(PFOS)对成人神经母瘤细胞(SH-SY5Y)的凋亡效应及可能的机制。方法实验中PFOS剂量设置为200、150、100、50、10、1 μmol/L和DMSO(对照组)。体外培养SH-SY5Y细胞,以不同浓度PFOS(1、10、50、100、150、200 μmol/L)处理细胞。染毒24 h和48 h后用CCK8法检测SH-SY5Y细胞活性,应用Annexin-V FITC/PI双染法结合流式细胞术检测细胞凋亡,紫外和荧光分光光度法检测SH-SY5Y细胞超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GSH)、总活性氧(ROS)和丙二醛(MDA)的影响。结果PFOS暴露降低了SH-SY5Y细胞活性,并呈明显的剂量效应关系；PFOS导致SH-SY5Y细胞凋亡；紫外和荧光分光光度法结果显示PFOS引起SH-SY5Y细胞中SOD和GSH水平下降,与对照组相比,最高剂量组PFOS引起GSH含量从8.00±0.12 nmol/mg蛋白降至5.89±0.90 nmol/mg蛋白；同时引起总活性氧(ROS)和丙二醛(MDA)水平上升,与对照组相比,最高剂量组PFOS引起MDA含量从1.92±0.17 nmol/mg蛋白显著上升至6.95±0.26 nmol/mg蛋白。结论PFOS可能通过对SH-SY5Y细胞产生氧化应激损伤从而对SH-SY5Y细胞产生毒性效应。 |
| 英文摘要: |
| Objective Explore the apoptosis effects of PFOS on SH-SY5Y cells and its mechanism.MethodsThe experiment was divided into seven groups,the control (DMSO) and the PFOS groups (1,0,50,0,150,0 μmol/L).The cell viability and time-efficiency of SH-SY5Y were determined by CCK8,the apoptosis rate were detected by Annexin-V FITC/PI double-staining,respectively.The effects of PFOS on oxidative stress damage in SH-SY5Y cells were detected by UV spectrophotometry and fluorescence methods.ResultsPFOS treatment significantly decreased SH-SY5Y cell viability in a dose dependent manner.The apoptosis effects were observed in SH-SY5Y cells obviously after PFOS exposure 48 h;The results of UV and fluorescence spectrophotometry showed that PFOS induced SH-SY5Y cell superoxide dismutase (SOD) and glutathione reductase (GSH) decreased,compared with the control,and the highest group of PFOS decreased GSH from 8±0.12 nmol/mg protein to 5.89±0.9 nmol/mg protein.While the total reactive oxygen species (ROS) and malondialdehyde (MDA) levels increased,compared with the control,the highest group of PFOS elevated MDA from 1.92±0.17 nmol/mg protein to 6.95±0.26 nmol/mg protein.ConclusionPFOS produced oxidative stress damage in SH-SY5Y cells,which may be the mechanisms of neurotoxicity induced by PFOS. |
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