常永梅,王明智,孙聪,颜文森,刘庆峰,江小运,王钧.AEG-1抑制miR-137对非小细胞肺癌增殖调控作用.[J].中南医学科学杂志.,2016,(3):279-282. |
AEG-1抑制miR-137对非小细胞肺癌增殖调控作用 |
AEG-1 Reverses the Inhibition of Non-small Cell Lung Cancer Proliferation Induced by MiR-137 |
投稿时间:2016-01-28 修订日期:2016-04-28 |
DOI: |
中文关键词: miR-137 非小细胞肺癌 胶质细胞上调基因1 增殖 |
英文关键词:miR-137 non-small cell lung cancer AEG-1 proliferation |
基金项目:广东省科技计划项目(2014A020212447). |
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中文摘要: |
目的 探讨AEG-1在miR-137抑制非小细胞肺癌(NSCLC)增殖中的作用及机制。方法收集2013年2月~2014年5月在本院手术治疗的65例NSCLC患者肿瘤标本,提取RNA,qRT-PCR检测NSCLC组织、A549、H460及16HBE细胞中miR-137与AEG-1的表达,统计分析NSCLC组织中miR-137与胶质细胞上调基因1(AEG-1)表达的相关性;将A549细胞根据不同转染试剂分成4组:转染miR-137 mimics及空载体组,转染miR-137 mimics及AEG-1表达载体组,转染miR-137 inhibitor及siRNA无关序列组,转染miR-137 inhibitor及AEG-1 siRNA组。用Lipofectamine 2000进行转染,生长曲线实验检测转染后细胞增殖情况。结果在NSCLC组织中miR-137与AEG-1的表达呈负相关;miR-137表达高的NSCLC细胞中AEG-1表达降低;共同转染miR-137 mimics与AEG-1过表达载体的A549细胞在48 h的细胞数高于对照组,而共同转染miR-137 inhibitor与AEG-1 siRNA的A549细胞在48 h、72 h的细胞数低于对照组。结论miR-137与AEG-1在NSCLC组织及细胞中表达呈负相关;AEG-1逆转miR-137对NSCLC细胞增殖的作用。 |
英文摘要: |
Objective To investigate the the function of AEG-1 (astrocyte elevated ) in the process of miR-137 inhibiting the proliferation of non-small cell lung cancer (NSCLC).MethodsSixty five samples in our hospital from Feb.2013 to May 2014 were collected.And RNA was extracted from these samples.QRT-PCR was conducted to detect the expression of miR-137 and AEG-1 in NSCLC tissues,and A549,H460 and 16HBE cells.The correlation between miR-137 and AEG-1 in NSCLC tissues was statistically analyzed.A549 cells were divided into four groups according to the transfection treatment:miR-137 mimics plus empty vector group,miR-137 mimics plus AEG-1 expression vector group,miR-137 inhibitor plus scramble sequence group,and miR-137 inhibitor plus AEG-1 siRNA group.The growth curve assay was performed to detect the cells proliferation after transfection treatment.ResultThe expression of miR-137 is negatively correlated with the AEG-1 in NSCLC tissues.The expression of AEG-1 was decreased in miR-137 overexpressed NSCLC cells.The cell number of A549 co-transfected with miR-137 mimics and AEG-1 expression vector was larger than the control group in 48 h.And,the cell number of A549 co-transfected with miR-137 inhibitor and AEG-1 siRNA was smaller than the control group in 48 h and 72 h.ConclusionThe expression of miR-137 is negatively correlated with the expression of AEG-1 in NSCLC tissues and cells;AEG-1 reversed the effect of miR-137 in the proliferation of NSCLC cell. |
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