| 石宇雄,郭智维,缪莉,谭志琴.miR-185与PKM2在胃癌中的表达及临床意义.[J].中南医学科学杂志.,2016,(3):267-270. |
| miR-185与PKM2在胃癌中的表达及临床意义 |
| Expression of miR-185 and PKM2 in Gastric Cancer andits Clinical Significance |
| 投稿时间：2016-01-28 修订日期：2016-04-27 |
| DOI： |
| 中文关键词: 胃癌 miR-185 PKM2 临床意义 |
| 英文关键词:gastric cancer miR-185 PKM2 clinical significance |
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| 中文摘要: |
| 目的 研究miR-185与PKM2在胃癌组织中的表达及其临床意义。方法采用原位杂交和免疫组化技术检测胃癌组织与癌旁正常胃粘膜组织中miR-185与PKM2的表达情况,分析miR-185与PKM2之间的相关性以及与胃癌患者临床病理参数的关系。结果原位杂交检测显示,在126例胃癌组织中miR-185阳性表达率为38.10%,与正常对照组56.38%比较,差异有统计学意义(χ2=14.36,P=0.000)。免疫组化结果显示,在126例胃癌组织中PKM2阳性表达率为63.49%,与正常对照组43.59%比较,差异有统计学意义(χ2=7.74,P=0.006)。相关性分析显示,在胃癌组织中miR-185的表达与PKM2的表达呈负相关(r=0.09,P=0.006)；Ⅲ~Ⅳ期胃癌患者miR-185的高表达率明显低于Ⅰ~Ⅱ期胃癌患者(P=0.001),且有淋巴结转移者miR-185的高表达率也明显低于无淋巴转移者(P=0.003)；而PKM2在Ⅲ~Ⅳ期胃癌患者的高表达率明显高于Ⅰ~Ⅱ期胃癌患者(P=0.002),且在有淋巴结转移者PKM2的高表达率也明显高于无淋巴转移者(P=0.006),但是miR-185与PKM2在胃癌患者中的表达与患者的年龄、性别、组织分化程度以及T分期无明显相关性。结论miR-185在胃癌组织中低表达,PKM2在胃癌组织中高表达可能是肿瘤细胞发生浸润转移的重要生物学标志。 |
| 英文摘要: |
| Objective To investigate the expression of miR-185 and PKM2 in gastric cancer and its clinical significance.MethodsIn situ hybridization and immunohistochemistry were used to detect the expression of miR-185 and PKM2 in gastric cancer and normal stomach tumour specimens,respectively.The correlations among the miR-185,PKM2,and clinicopathological parameters of gastric cancer patients were analyzed.ResultsIn situ hybridization showed that the positive rate of miR-185 was 38.10% in gastric cancer,and 65.38% in normal stomach tumour specimens,the difference was statistically significant (χ2=14.36,P=0.000).Immunohistochemistry showed that the positive rate of PKM2 was 63.49% in gastric cancer,and 43.59% in normal stomach tumour specimens,the difference was statistically significant (χ2=7.74,P=0.006).The correlation analysis indicated that the expression of miR-185 had a negative correlation with that of PKM2 in gastric cancer(P<0.01).The positive rate of miR-185 in Ⅲ~Ⅳ stage gastric cancer was strongly lower than in Ⅰ~Ⅱ stage(P=0.001),the expression of miR-185 was also lower with lymph node metastasis than without lymph node metastasis in gastric carcinoma(P=0.003).The positive rate of PKM2 in Ⅲ~Ⅳ stage gastric cancer was strongly higher than in Ⅰ~Ⅱstage(P=0.002),the expression of PKM2 was also higher with lymph node metastasis than without lymph node metastasis in gastric carcinoma(P=0.006).However,there were no significant difference between different age,gender,histological grade as well as T stage group (P>0.05).ConclusionThe low expression of miR-185 and the high expression of the PKM2 protein may be important biological markers for malignant transformation in gastric cancer. |
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