| 刘晓旺,陈霄霄,周志刚,熊婷,李丽丁,陈雪艳,彭露,涂剑.二烯丙基二硫下调uPA和MMP9抑制乳腺癌细胞侵袭迁移.[J].中南医学科学杂志.,2016,(2):125-129. |
| 二烯丙基二硫下调uPA和MMP9抑制乳腺癌细胞侵袭迁移 |
| Diallyl Disulfide Inhibits the Invasion and Migration of BreastCancer Cells Down-regulating uPA and MMP9 |
| 投稿时间：2015-11-21 修订日期：2016-02-28 |
| DOI： |
| 中文关键词: 二烯丙基二硫 人乳腺癌细胞 细胞侵袭 细胞迁移 |
| 英文关键词:diallyl disulfide (DADS) human breast cancer cells cell invasion cell migration |
| 基金项目:国家级大学生创新创业训练计划项目(201410555008)和湖南省大学生创新性课题(2014-234);湖南省中医药局课题(2014144);南华大学研究生创新性课题(2015XCX33);“湖南省分子靶标新药研究协同创新中心”培育项目(2014-405)和湖南省“十二五”重点学科建设项目. *:并列第一作者. * |
|
| 摘要点击次数: 642 |
| 全文下载次数: 720 |
| 中文摘要: |
| 目的 研究大蒜提取物二烯丙基二硫(DADS)对MCF-7和MDA-MB-231两种人乳腺癌细胞侵袭迁移的作用。方法采用不同浓度(0,0,200,0 μmol/L)DADS处理MCF-7和MDA-MB-231两种人乳腺癌细胞24 h,Transwell侵袭实验检测DADS对细胞侵袭能力的影响；划痕愈合实验观察DADS对细胞迁移能力的改变；western blot检测两种细胞中尿激酶型纤溶酶原激活剂(uPA)和基质金属蛋白酶9(MMP9)的表达。结果Transwell实验发现DADS呈浓度依赖性抑制MCF-7和MDA-MB-231两种乳腺癌细胞的侵袭,划痕愈合实验则发现DADS呈浓度依赖性抑制两种乳腺癌细胞的迁移。与此同时,Western blot结果显示DADS可浓度依赖性下调侵袭迁移相关蛋白uPA和MMP9的表达。结论DADS可下调uPA和MMP9表达,抑制乳腺癌细胞侵袭迁移。 |
| 英文摘要: |
| Objective To explore the role of diallyl disulfide (DADS) on the invasion and migration of human breast cancer cells.MethodsMCF-7 and MDA-MB-231 human breast cancer cells were in vitro treated with different concentration (0,0,200,0 μmol/L) of DADS for 24h.Then,Transwell chamber assay and wound healing assay were used to detect the ability of cell invasion/migration.The protein expression of uPA and MMP9 were detected by western blot.ResultsDADS inhibited the invasion and migration of MCF-7 and MDA-MB-231 cells in a dose-dependent manner.At the same time,the invasion and migration-related protein expression of uPA and MMP9 were both down-regulated.ConclusionDADS could inhibit the invasion and migration of MCF-7 and MDA-MB-231 cells by down-regulating the protein expression of uPA and MMP9. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|