| 肖子建， 谢 明， 周桂娟， 兰 芳， 邓 琦.姜黄素以端粒酶为靶点治疗Aβ损伤的体外研究.[J].中南医学科学杂志.,2015,43(6):618-621. |
| 姜黄素以端粒酶为靶点治疗Aβ损伤的体外研究 |
| Telomerase:a Target for Therapeutic Effects of Curcumin and a Curcumin Derivative in Aβ Insult in vitro |
| 投稿时间：2015-07-26 |
| DOI： |
| 中文关键词: β淀粉样蛋白 姜黄素 端粒酶 人端粒酶逆转录酶 |
| 英文关键词:amyloid-beta curcumin telomerase human telomerase reverse transcriptase |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨端粒酶在姜黄素治疗β淀粉样蛋白（Aβ）损伤的神经保护效应中的作用。方法 用Aβ1-42（10 μg/mL）预处理SK-N-SH细胞建立损伤的细胞模型后，用姜黄素（10 μg/mL）干预治疗组，处理前后检测细胞存活率、细胞内氧化应激水平及hTERT的表达水平，并通过RNA干扰技术降低hTERT的表达，以此验证端粒酶在姜黄素的干预作用中所起的作用。结果 Aβ1-42预处理组的氧化应激水平及细胞毒性均有显著增高（P＜0.001），hTERT的表达水平明显下降（P＜0.001）。姜黄素干预使SK-N-SH细胞免受Aβ1-42损伤（P＜0.001）并上调hTERT的表达水平（P＜0.001）。而当端粒酶活性被TERT的小分子干扰RNA抑制后，姜黄素的神经保护作用也随之消失（P＞0.05）。结论 姜黄素对抗Aβ毒性的神经保护作用有赖于端粒酶活性，端粒酶则可能是姜黄素治疗效应的靶点。 |
| 英文摘要: |
| Objective To investigate whether telomerase was involved in the neuroprotective effect of curcumin against Aβ. Methods Aβ1-42 （10 μg/mL） was used to establish a damaged cell model and curcumin （10 μg/mL） was used in treatment groups.Cell survival and cell growth,intracellular oxidative stress and hTERT expression was measured.After RNA interference,the effects of curcumin on cells were verified. Results Exposure to Aβ1-42 resulted in significant oxidative stress and cell toxicity,and the expression of hTERT was significantly decreased （P＜0.001）.Curcumin protected SK-N-SH cells from Aβ1-42 and up-regulated the expression of hTERT （P＜0.001）.However,when telomerase activity was inhibited by TERT siRNA,the neuroprotection by curcumin was whittled （P＞0.05）. Conclusion The neuroprotective effect of curcumin against Aβ depends on telomerase activity,and thus telomerase may be a target for therapeutic effect of curcumin. |
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