| 谢雪姣，李亚梅，徐 佳，廖端芳，吴若霞，尹栋梁，陈聪伶.加味二陈汤对ApoE-/-小鼠炎症标志物IL-6和TNF-α的调节作用.[J].中南医学科学杂志.,2015,43(6):605-609. |
| 加味二陈汤对ApoE-/-小鼠炎症标志物IL-6和TNF-α的调节作用 |
| The Effects of Modified “Erchen Decoction”on the Expression of Inflammatory Marker IL-6，TNF-α on ApoE-/- Mice |
| 投稿时间：2015-08-25 |
| DOI： |
| 中文关键词: 加味二陈汤 动脉粥样硬化 TNF-α IL-6 |
| 英文关键词:Modified Erchen Decoction atherosclerosis TNF-α IL-6 |
| 基金项目:全国第五批老中医药专家学术经验继承项目，湖南省教育厅科研青年项目（编号11B092）湖南省中医药科研计划项目（编号201236）湖南省自然基金项目（编号：2015JJ6076）湖南省中药粉体与创新药物省部共建国家重点实验室培育基地资助 |
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| 中文摘要: |
| 目的 研究加味二陈汤对ApoE-/-小鼠血清、主动脉壁及瓣膜的IL-6、TNF-α表达的影响。方法 40只ApoE-/-小鼠中10只为空白对照组，30只以高脂饲喂养4周构建动脉粥样硬化（As）小鼠模型，均分成模型组、辛伐他汀组、加味二陈汤组，分别用生理盐水、辛伐他汀及加味二陈汤对小鼠灌胃给药，8周后处死小鼠，Elisa法检测血清中IL-6、TNF-α的含量；免疫组化法检测IL-6、TNF-α在主动脉壁及瓣膜的表达。结果 辛伐他汀组血清IL-6和TNF-α分别为392.06±37.70 pg/mL、189.1±50.4 pg/mL，与模型组相比表达下降（P<0.05）；加味二陈汤组血清IL-6、TNF-α分别为442.41±172.26 pg/mL、47.2±13.3 pg/mL，与模型组相比表达下降（P<0.05），与辛伐他汀组相比，表达下降（P<0.05）；免疫组化结果显示加味二陈汤可降低IL-6、TNF-α在主动脉壁内的表达。结论 加味二陈汤可降低血清和主动脉壁及瓣膜的IL-6、TNF-α的表达。 |
| 英文摘要: |
| Objective This study was to investigate the effects of Modified Erchen Decoction on the expression of IL-6，TNF-α in the aortic wall and serum of ApoE-/- mice，in order to confirm the effect on the regulation of inflammatory factors in atherosclerosis. Methods ApoE-/- mice of the control group （n10） were fed with normal diet,the others （n30） were fed with a high-cholesterol diet in 4 weeks to induce atherosclerosis mode,and then were divided into the model group （n10）,the Modified Erchen Decoction group （n10） and the simvastatin group （n10）.The two treatment groups were given Modified Erchen Decoction and simvastatin by gavage respectively.The expression of IL-6 and TNF-α was measured by ELISA kit after treated with the drugs for 8 weeks continuously. Results IL-6 and TNF-α in serum were 392.06±37.70 pg/mL and 189.1±50.4 pg/mL in the simvastatin group and were 47.2±13.3 pg/mL and 442.41±172.26 pg/mL in the Modified Erchen Decoction group respectively.They were decreased by the two drugs,and significant difference was found between each treatment group and the control group.The expression of IL-6 and TNF-α were decreased by the two drugs,which were confirmed by the histopathology. Conclusion Modified Erchen Decoction can inhibit the expression of IL-6 and TNF-α effectively in the ApoE-/- mice,which indicates that Chinese formula may reduce atherosclerosis progress. |
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