| 刘迪群，温彩玲，钟 华，周伟伟.Kiss-1高表达抑制结肠癌侵袭与转移能力的影响.[J].中南医学科学杂志.,2015,43(5):510-514. |
| Kiss-1高表达抑制结肠癌侵袭与转移能力的影响 |
| The Effect of Kiss-1 High Expression On Inhibiting Invasion and Metastasis of Colon Cancer |
| 投稿时间：2015-05-11 |
| DOI： |
| 中文关键词: 结肠癌SW480细胞 Kiss-1 MMP-9 MMP-2 侵袭 转移 |
| 英文关键词:SW480 colon cancer cells Kiss-1 MMP-9 MMP-2 invasion metastasis |
| 基金项目:湖南省教育厅一般项目（11C1107）. |
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| 中文摘要: |
| 目的 探讨Kiss-1高表达对结肠癌SW480细胞侵袭与转移能力的影响，初步明确Kiss-1基因对基质金属蛋白酶-9（matrix metalloproteinase-9,MMP-9）和MMP-2表达的影响。 方法 用脂质体转染方法将质粒pEGFP-N1-Kiss-1及pEGFP-N1空载体质粒瞬时导入结肠癌SW480细胞，细胞随机分三组：空白对照组、阴性对照组、实验组。应用Transwell实验检测结肠癌细胞的侵袭能力、Western-blot和Real-time PCR分别从蛋白和mRNA水平检测Kiss-1表达对MMP-9、MMP-2表达的影响。 结果 成功将pEGFP-N1-Kiss-1重组质粒瞬时导入结肠癌SW480细胞。Transwell实验检测出实验组较阴性对照组和空白对照组能明显降低SW480细胞侵袭能力的作用（P<0.05）。Western blot和Real-time PCR检测出实验组Kiss-1 蛋白和mRNA表达水平较阴性对照组或空白对照组显著增加，而实验组MMP-9、MMP-2蛋白和mRNA表达水平显著降低，其差异均具有统计学意义（P<0.05）。 结论 Kiss-1抑制MMP-9和MMP-2的表达，其调控机制可能与抑制结肠癌的侵袭和转移能力有关。 |
| 英文摘要: |
| Objective To explore the effect of Kiss-1 high expression on invasion and metastasis of the colon cancer SW480 cells.To preliminarily define the influence of kiss-1 genes to the expressions of matrix metalloproteinase-9（MMP-9） and MMP-2. Methods To guide plasmids pEGFP-N1-Kiss-1 and pEGFP-N1 into the colon cancer SW480 sells instantly with the method of lipofectin transfection.Divide cells into three groups:the blank control group with no transfection cell,the negative group with transfection of the empty pEGFP-N1 cell,the experimental group with transfection of plasmid pEGFP-N1-Kiss-1 cell and up-regulation Kiss-1 expression level in colon cancer cells.Applicate Transwell experiment to detect the invasion ability of the colon cancer cells.Western blot and Real-time PCR,respectively from protein and the level of mRNA,were used to detect the influence of Kiss-1 expression to the expressions of MMP-9 and MMP-2. Results Successfully guided pEGFP-N1-Kiss-1 recombinant plasmid into the colon cancer SW480 sells.Transwell experiments detected that the experimental group,compared with the negative control group and the blank control group,can obviously reduce SW480 cells invasion ability （P<0.05）.Western blot and Real-time PCR detected that the experimental group Kiss-1 protein and mRNA expression level increased significantly,while MMP-9,MMP-2 protein and mRNA expression level decreased significantly,compared with negative control group or blank control group,the differences were statistically significant （P<0.05）. Conclusion Kiss-1 can inhibit the expressions of MMP-9 and MMP-2.Its regulatory mechanism may be related to its inhibition to the invasion and metastasis ability of colon cancer. |
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