| 胡 璟，高春生，刘 林，杜政德.氧化/抗氧化失衡在大鼠中枢听觉系统老化过程中的作用.[J].中南医学科学杂志.,2015,43(5):502-505. |
| 氧化/抗氧化失衡在大鼠中枢听觉系统老化过程中的作用 |
| The Role of Oxidation/Antioxidant Imbalance in the Age-related Damage of the Rat Central Auditory System |
| 投稿时间：2015-01-20 |
| DOI： |
| 中文关键词: 氧化/抗氧化失衡 老化 中枢听觉系统 氧化性损伤 线粒体DNA |
| 英文关键词:oxidation/antioxidant imbalance aging central auditory system oxidative damage mitochondrial DNA |
| 基金项目:广东省医学科研基金（B2014370）；深圳市科技计划（JCYJ20140411092351692）；深圳市南山区技术研发和创意设计项目分项资金（南科研卫2012014号）. |
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| 中文摘要: |
| 目的 研究氧化/抗氧化失衡在D-半乳糖诱导的大鼠老化中枢听觉系统听皮层组织中的作用，探讨老年性耳聋氧化性损伤的发生机制。 方法 48只1月龄雄性Spragua-Dawley大鼠随机分成4组（各12只），不同剂量D-半乳糖每日颈背部皮下注射D-半乳糖（500 mg/kg）造模，连续8周。造模完成后，取4组大鼠中枢听觉系统听皮层组织，检测活性氧指标过氧化氢、总抗氧化能力指标抗氧化大分子、抗氧化小分子和酶的总水平、DNA氧化损伤生物标记物8-羟基-2-脱氧鸟苷（8-OHdG）的表达以及最常发生的年龄相关性mtDNA损伤线粒体DNA（mtDNA）4 834 bp大片段缺失的累积水平。将实验数据采用方差分析进行分析。 结果 与对照组大鼠相比较，D-半乳糖诱导的老化大鼠听皮层组织中过氧化氢含量明显增多，而总抗氧化能力明显下降（P<0.01）。同时，DNA氧化损伤的产物8-OHdG的表达和线粒体4 834 bp大片段缺失突变的累积明显增多（P<0.01）。 结论 在中枢听觉系统老化过程中，氧化/抗氧化失衡可能是导致老年性耳聋发生的重要原因。 |
| 英文摘要: |
| Objective To explore the effects of oxidation/antioxidant imbalance on the auditory cortex of central auditory system of D-galactose-induced aging rats and investigate the mechanism of oxidative damage of age-related hearing loss. Methods Forty-eight 1-month male Sprague-Dawley rats were randomly divided into four groups （12 rats in each group），and were injected subcutaneously with different dose of D-gal once a day for 8 weeks.After the experiment termination,the tissues were harvested from the auditory cortex of central auditory system.Investigate the expression of hydrogen peroxide,total antioxidant capacity,8-hydroxy-2-deoxyguanosine （8-OHdG） and the accumulation of mitochondrial DNA 4 834 bp deletion.All experimental data was analysed with one-way ANOVA. Results The expression of hydrogen peroxide in the auditory cortex of rats of D-galactose groups was significantly increased compared with the control group,while the expression of the total antioxidant capacity was significantly decreased （all P<0.01）.Meanwhile,the expression of 8-OHdG and the accumulation of mitochondrial DNA 4 834 bp deletion were significantly increased （P<0.01）. Conclusions In the aging process of central auditory system,the oxidation/antioxidant imbalance may be an important cause for the age-related hearing loss. |
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