| 向姝霖，刘 芳，夏 红，曾 希，苏 波，董 琳，凌 晖，苏 琦.二烯丙基二硫下调β-catenin抑制人胃癌MGC803细胞EMT.[J].中南医学科学杂志.,2015,43(5):487-492. |
| 二烯丙基二硫下调β-catenin抑制人胃癌MGC803细胞EMT |
| DADS Inhibit EMT Through Down-Regulation of β-catenin in Human Gastric Cancer MGC803 Cells |
| 投稿时间：2015-07-30 |
| DOI： |
| 中文关键词: 二烯丙基二硫 人胃癌MGC803细胞 EMT β-catenin E-cadherin Vimentin |
| 英文关键词:diallyl disulfide human gastric cancer MGC803 cells EMT β-catenin E-cadherin Vimentin |
| 基金项目:国家自然科学基金（81102854,31100935，81374013）；湖南省卫计委科研项目（B2015-182）. |
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| 中文摘要: |
| 目的 研究二烯丙基二硫（DADS）抑制人胃癌MGC803细胞EMT与形态学变化。 方法 应用相差显微镜与透射电镜观察30 mg·L-1 DADS处理后的MGC803细胞形态学与超微结构的变化。RT-PCR与Western blot检测β-catenin和EMT标记E-cadherin与Vimentin表达。 结果 30 mg·L-1 DADS处理MGC803细胞24 h后，相差显微镜显示，与未处理比较，细胞圆形、椭圆形，核浆比值降低，巢状分布，均未见伪足。透射电镜显示，细胞表面微绒毛数目减少，细胞核变规整，核浆比值下降，异型性降低，细胞与细胞之间出现连接结构，细胞器增多。RT-PCR与Western blot表明，DADS可呈时间依赖性分别下调MGC803细胞β-catenin和Vimentin mRNA与蛋白和上调E-cadherin mRNA与蛋白。 结论 DADS可通过下调β-catenin上调E-cadherin与下调Vimentin抑制EMT和伪足形成。 |
| 英文摘要: |
| Objective To investigate the effect of EMT and morphology change in human gastric cancer MGC803 cells induced by diallyl disulfide （DADS）. Methods The phase contrast microscope and transmission electron microscope were employed to confirm the DADS-induced morphology change.The expression of β-catenin,E-cadherin and Vimentin was detected by RT-PCR and Western blot in MGC803 cells after treated by 30 mg·L-1 DADS. Results The phase contrast microscope show that MGC803 cells after 24 h treated by 30 mg·L-1 DADS were round or ellipse,enlarged cytoplasm,descend nuclear/cytoplasmic ratio,exhibited nest and even not view pseudopodia.Transmission electron microscope results show,that the number of microvilli on cells surface decrease,malignance declining as cellular heteromorphism diminish,nucleocytoplasmic proportion reduce,intercellular conjunctional structure appear and cellular organ increase in cytoplasm after MGC803 cells treated by DADS to contrast untreated.RT-PCR and Western blot indicated that DADS may be down-regulation of β-catenin and Vimentin mRNA and protein up-regulation of E-cadherin mRNA and protein in time dependence in MGC803 cells. Conclusion All these suggested that DADS may inhibit EMT and pseudopodia via down-regulation of β-catenin to up-regulate E-cadherin and down-regulate Vimentin in MGC803 cells. |
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