| 刘 健，吴 洁，李群芳，罗 文.阿托伐他汀对血脂异常兔血清和脂肪.[J].中南医学科学杂志.,2015,43(4):388-392. |
| 阿托伐他汀对血脂异常兔血清和脂肪 |
| The Influences of Atorvastatin on Levels of Adiponectin in Serum and Adipose Tissue of Dyslipidemia Rabbits |
| 投稿时间：2014-09-11 |
| DOI： |
| 中文关键词: 血脂异常兔 阿托伐他汀 脂联素 过氧化物酶增殖体激活受体γ |
| 英文关键词:dyslipidemia rabbit atorvastatin adiponectin peroxisome proliferation activated receptor gamma |
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| 中文摘要: |
| 目的 观察阿托伐他汀干预下血脂异常兔血清脂联素的水平以及脂肪组织过氧化物酶增殖体激活受体γ（PPARγ）和脂联素分泌量的变化，推测阿托伐他汀抗动脉粥样硬化的可能机制。 方法 将15只纯种新西兰大白兔随机分为3组进行血脂造模。造模结束后，留取兔主动脉标本并切片做HE染色，观察动脉粥样硬化斑块的变化；取腹股沟皮下脂肪组织称量，并进行脂肪组织孵育；应用逆转录聚合酶链反应技术测定脂肪细胞过氧化物酶增殖体激活受体mRNA和脂联素mRNA的表达；酶联免疫吸附试验测定血清及腹股沟脂肪细胞提取液中脂联素浓度。 结果 阿托伐他汀组兔主动脉粥样斑块面积明显低于高胆固醇组（P<0.05）；高胆固醇组兔血清低密度脂蛋白胆固醇、血清总胆固醇浓度明显高于对照组（P＜0.05）；阿托伐他汀药物治疗后能明显降低兔血清低密度脂蛋白胆固醇以及总胆固醇浓度（P＜0.05）；高胆固醇组兔血清脂联素浓度和脂肪组织分泌的脂联素浓度较对照组低（P＜0.05）；阿托伐他汀药物治疗后能明显升高血清脂联素浓度并提高脂肪组织分泌脂联素的浓度（与高胆固醇组比较，P＜0.05）；血清脂联素浓度与脂肪组织分泌的脂联素浓度呈正相关（P＜0.05）；RT-PCR结果显示高胆固醇组脂肪组织过氧化物酶增殖体激活受体mRNA和脂联素mRNA表达低于对照组（P＜0.05）；阿托伐他汀组脂肪组织过氧化物酶增殖体激活受体mRNA和脂联素mRNA的表达高于高胆固醇组（P＜0.05）。 结论 阿托伐他汀能上调血脂异常兔脂肪组织过氧化物酶增殖体激活受体γ和脂联素mRNA表达从而升高血清和脂肪组织脂联素的分泌水平，这可能是阿托伐他汀抗动脉粥样硬化作用的多效性机制之一。 |
| 英文摘要: |
| Objective To observe the changes of serum adiponectin（APN）level,peroxisome proliferation activated receptor gamma（PPARγ） and APN secretion in adipose tissues of dyslipidemia rabbits under atorvastatin intervention and to elaborate the possible anti-atherosclerosis mechanism of atorvastatin. Methods Fifteen pure New Zealand white rabbits were randomly divided into three groups to make lipid models.Then the Aortic specimens were obtained and sectioned to make HE staining and the atherosclerotic plaque changes were observed.The inguinal subcutaneous adipose tissues were obtained and weighed and incubation of adipose tissues was made.Reverse transcriptase polymerase chain reaction （RT-PCR） technology was applied to determine the expression of peroxisome proliferation activated receptor mRNA and adiponectin mRNA in fat cells.Enzyme linked immunosorbent assay was applied to determine the concentration of serum and the adiponectin in the extract from the inguinal fat cells. Results The aortic atherosclerotic plaque area in the rabbits of the atorvastatin group was significantly lower than that in the rabbits of the high cholesterol group（P<0.05）.The concentration of the low density lipoprotein cholesterol （LDL-C） and the total cholesterol （TC） in the serum of the rabbits of the high cholesterol group was significantly higher than that in the contrast group（P<0.05）.Atorvastatin drug intervention for 4 weeks can significantly reduce the concentration of the low density lipoprotein cholesterol （LDL-C） and the total cholesterol （TC） in the serum of the rabbits （compared before and after the treatment,P<0.05）.Serum APN concentration and the concentration of the APN secreted from adipose tissues in the rabbits of the high cholesterol group were lower than those in the contrast group （P<0.05）.Atorvastatin drug intervention could significantly raise serum APN concentration and the concentration of the APN secreted from adipose tissues （compared with high cholesterol group,P<0.05）.The serum APN concentration and the concentration of the APN secreted from adipose tissues were positively correlated（P＜0.05）.RT-PCR results showed that the expression of peroxisome proliferation activated receptor mRNA and adiponectin mRNA in fat cells of the rabbits in the high cholesterol group was lower than that in the contrast group（P<0.05） and the expression of peroxisome proliferation activated receptor mRNA and adiponectin mRNA in the atorvastatin group was higher than that in the high cholesterol group（P<0.05）. Conclusion Atorvastatin increases the expression of peroxisome proliferation activated receptor gamma（PPARγ）and adiponectin mRNA in the adipose tissues of dyslipidemia rabbits,thus improving the secreting level of serum and adiponectin in fat cells,which may be one of the pleiotropic anti-atherosclerotic mechanisms of atorvastatin. |
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