| 何 璐，游晓星，李国华，曾焱华，李冉辉，朱翠明，余敏君，陈忠东，吴移谋.生殖支原体脂质相关膜蛋白通过c-Src/ROS/Nrf2途径.[J].中南医学科学杂志.,2015,43(4):364-368. |
| 生殖支原体脂质相关膜蛋白通过c-Src/ROS/Nrf2途径 |
| Mycoplasma Genitalium-derived Lipid-associated Membrane Proteins Induces Placental Trophoblast Cells Expression of HO-1 via ROS/Nrf2 |
| 投稿时间：2015-04-24 |
| DOI： |
| 中文关键词: 生殖支原体 脂质相关膜蛋白 血红素氧合酶-1 滋养层细胞 |
| 英文关键词:mycoplasma genitalium lipid-associated membrane proteins heme oxygenase-1 placental trophoblast cells |
| 基金项目:湖南省自然科学衡阳联合基金（12JJ9033）；湖南省自然科学基金（12JJ3102、13JJ3079）；湖南省科技厅项目（2013SK3118、2014SK3081）；湖南省高校创新平台基金（12K094、13K082、13K083）；湖南省教育厅课题（11C1112、12C0340）；衡阳市科技局项目（2013KJ12、2013KJ28）；邵阳市科技局重点项目（2013SK13）；湖南省卫生厅项目（B2013-048、B2014-163）. |
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| 中文摘要: |
| 目的 探讨生殖支原体脂质相关膜蛋白（LAMPs）诱导胎盘滋养层细胞表达血红素氧合酶-1（HO-1）的分子机制。 方法 用0.5～5 μg/mL生殖支原体LAMPs处理体外培养的胎盘滋养层细胞4～12 h，采用实时定量PCR和Western blot法分别检测HO-1 mRNA和蛋白的表达以及核因子相关因子-2（Nrf2）的核转位；比色法观察HO-1的酶活性；2′,7′-二氯二氢荧光黄二乙酸酯（H2DCFDA）检测活性氧产生。分别采用酪氨酸激酶c-Src抑制剂PP1、活性氧抑制剂N-乙酰半胱氨酸（NAC）和Nrf2 siRNA干预，观察HO-1的表达情况。 结果 生殖支原体LAMPs能诱导滋养层细胞HO-1 mRNA和蛋白的表达，上调其酶活性。同时，LAMPs也能诱导其产生活性氧，并促使Nrf2核转位。PP1和NAC预处理后，可明显降低HO-1的表达水平以及细胞核内Nrf2含量。采用Nrf2 siRNA转染后，HO-1的表达显著减少。 结论 生殖支原体LAMPs通过c-Src/ROS/Nrf2途径诱导滋养层细胞表达HO-1。 |
| 英文摘要: |
| Objective To investigate the mechanism of the expression of heme oxygenase-1 （HO-1） in response to Mycoplasma genitalium-derived lipid-associated membrane proteins （LAMPs） in placental trophoblast cells. Methods Placental trophoblast cells were cultured in vitro and stimulated by 0.5～5 μg/mL of LAMPs for 4～12h,expression of HO-1 mRNA and protein,and nuclear translocation of nuclear factor erythroid-2 related factor 2 （Nrf2） were detected by real-time PCR and Western blot,respectively.The enzyme activity of HO-1 was detected by colorimetric method.The intracellular formation of reactive oxygen species （ROS） was detected by using the fluorescent probe H2DCFDA.Tyrosine kinase c-Src inhibitor PP1,N-acetyl-cysteine （NAC） and Nrf2 siRNA were used to analyse the function of ROS and Nrf2 in mediating of HO-1 expression. Results M.genitalium LAMPs enhanced the expression of HO-1 at mRNA and protein levels as well as the enzyme activity of HO-1 in placental trophoblast cells in a concentration-dependent manner.In the meantime,LAMPs also induced placental trophoblast cells accumulation of ROS and nuclear translocation of Nrf2.PP1 and NAC treatment could inhibit LAMPs-induced HO-1 expression and Nrf2 nuclear translocation,and transfection of Nrf2 siRNA significantly abrogate HO-1 expression. Conclusion M.genitalium LAMPs induced placental trophoblast cells expression of HO-1 through c-Src/ROS/Nrf2 pathways. |
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