| 吴 广，曾赛丽.HPLC联合UV法测定烟酸片中烟酸的含量.[J].中南医学科学杂志.,2015,43(3):319-321. |
| HPLC联合UV法测定烟酸片中烟酸的含量 |
| Determination of the Nicotinic Acid in Nicotinic Acid Tablets by HPLC Combined with UV |
| 投稿时间：2014-12-24 |
| DOI： |
| 中文关键词: HPLC UV 含量测定 质量控制 烟酸 |
| 英文关键词:HPLC UV content determination quality control nicotinic acid |
| 基金项目: |
|
| 摘要点击次数: 957 |
| 全文下载次数: 1466 |
| 中文摘要: |
| 目的 建立一种精确可靠的HPLC/UV法用于测定烟酸片中烟酸的含量。方法：HPLC法采用Aglient C18色谱柱，以0.02 mol/L磷酸二氢钾-乙腈（90∶10）为流动相等度洗脱，流速1.0 mL/min，检测波长263 nm；UV法以磷酸二氢钾-乙腈作为溶剂，在 263 nm 波长处测定吸光度；并通过检测其线性范围、精密度、准确度和加样回收率对该方法进行验证。结果： 烟酸检测质量浓度在5.0～100.0μg/mL 范围内，与吸光度和峰面积积分值呈良好的线性关系，UV法检测r0.9959，平均加样回收率为99.57%，RSD1.08%（n6）；HPLC法检测r0.999 8，平均加样回收率为99.62%，RSD0.74%（n6）。结论：HPLC法和UV法联用操作简捷、准确、重复性好，并且具有更好的专属性，可用于烟酸片烟酸的含量测定和质量控制。 |
| 英文摘要: |
| Objective To establish an accurate and reliable HPLC/UV method for the quantitative determination of nicotinic acid in nicotinic acid tablets. Methods The chromatographic separation conditions employed for HPLV method were chosen using Aglient C18 column.0.02 mol/L potassium dihydrogen phosphate （Ph 6.8）-acetonitrile （90∶10） were used as the mobile phase pumped at a flow rate of 1.0mL/min and a detection wavelength at 263 nm was used.UV method use potassium dihydrogen phosphate （Ph 6.8）-acetonitrile （90∶10） as solvent,and the detection wavelength were at 263 nm.The linearity,precision,accuracy,recovery rate and other content were tested to validate the method. Results The linear range of nictinic acid was 5.0～100.0 μg/mL.With UV method （r0.995 9）,the average recovery is 99.57% （RSD1.08%,n6）with HPLC method （r0.999 8）,the average recovery is 99.62% （RSD0.74%,n6）. Conclusion The HPLC/UV method is simple,accurate,high-specificial and reproducible.This method can be used for the quality control nicotinic acid tablets. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |