| 唐群华，孙晓红.紫杉醇对Caski细胞增殖及APC、DKK-3基因表达的影响.[J].中南医学科学杂志.,2015,43(3):257-261. |
| 紫杉醇对Caski细胞增殖及APC、DKK-3基因表达的影响 |
| Effects of Paclitaxel on Cell Proliferation and Expression of APC and DKK-3 in Human Cervical Cancer Caski Cells |
| 投稿时间：2015-03-01 |
| DOI： |
| 中文关键词: 宫颈癌 紫杉醇 启动子甲基化 APC DKK-3 |
| 英文关键词:cervical cancer paclitaxel promoter methylation APC DKK-3 |
| 基金项目: |
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| 中文摘要: |
| 目的 研究紫杉醇对Caski细胞增殖及APC、DKK-3基因表达的影响。 方法 用MTT法、RT-PCR法及甲基化特异性PCR分别检测紫杉醇对人宫颈癌Caski细胞株增殖的影响、APC与DKK-3 mRNA 表达水平、APC与DKK-3启动子甲基化水平。 结果 ≥1 μmol/L紫杉醇能明显抑制人宫颈癌Caski细胞株的增殖 （P<0.05）；从5 μmol/L开始，随着紫杉醇给药剂量的增大，Caski细胞APC、DKK-3 mRNA表达水平明显增加 （P<0.05），而Caski细胞APC 、DKK-3启动子甲基化水平显著降低 （P<0.05）。 结论 紫杉醇能呈剂量依赖性的抑制Caski 细胞的增殖并上调APC、DKK-3 mRNA的表达，这可能与其诱导APC、DKK-3启动子的去甲基化有关。 |
| 英文摘要: |
| Objective To study the effect of paclitaxel on the proliferation of Caski cells and the expression of APC,DKK-3 gene. Methods MTT colorimetric method,RT-PCR method and methylation specific PCR were used to detect the proliferation effects,the mRNA expression level of APC and DKK-3,APC and DKK-3 promoter methylation level induced by paclitaxel on human cervical carcinoma Caski cell line,respectively. Results Paclitaxel significantly inhibited the proliferation of Caski cervical cancer cell line when the concentration of Paclitaxel was more than 1 μmol/L （P<0.05）Starting from concentration of 5 μmol/L,with increasing of paclitaxel dosage,APC and DKK-3 mRNA expression level increased significantly （P<0.05）,while APC and DKK-3 promoter methylation level significantly decreased （P<0.05）. Conclution Paclitaxel could dose-dependently inhibit the proliferation of Caski cells through increasing APC and DKK-3 mRNA expression,which may be related with the inhibition of APC and DKK-3 promoter methylation. |
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