| 李 熠，匡稳定，周容容，桂庆军，匡双玉.山银花黄酮类提取物对人肝L-02细胞甘油三酯蓄积及SREBP-1c表达的影响.[J].中南医学科学杂志.,2015,43(2):145-149. |
| 山银花黄酮类提取物对人肝L-02细胞甘油三酯蓄积及SREBP-1c表达的影响 |
| Effects of Flavonoid Extract from Flos Lonicerae Confusae on Triglyceride Accumulation and SREBP-1c Expression in Human Hepatocyte |
| 投稿时间：2014-04-24 |
| DOI： |
| 中文关键词: 山银花 提取物 黄酮 TG SREBP-1c |
| 英文关键词:Lonicerae Confusae extracts flavone triglyceride SREBP-1c |
| 基金项目:本课题受衡阳市2012年科学技术发展计划项目（No.2012KJ8）及2013年湖南省教育厅优秀青年科研项目（2013B098）资助. |
|
| 摘要点击次数: 696 |
| 全文下载次数: 899 |
| 中文摘要: |
| 目的 观察山银花黄酮类提取物对人肝L-02细胞甘油三酯蓄积的影响，并初步探讨SREBP-1c蛋白及基因表达在其中的作用。 方法 油红O染色初步观察山银花黄酮类提取物对脂变人肝L-02细胞中脂滴形成的影响。然后以脂变人肝L-02细胞为模型，不同浓度（0、5、10、20 mg/L）山银花黄酮类提取物作用于细胞24 h以及10 mg/L山银花黄酮类提取物作用于细胞不同时间（0、12、24、48、72 h），生化试剂盒测定细胞内甘油三酯（TG）的含量，Western印迹法检测细胞内SREBP-1c蛋白表达变化，实时荧光定量PCR检测细胞内SREBP-1c mRNA表达变化。 结果 山银花黄酮类提取物显著降低人肝L-02细胞中脂滴含量，细胞内TG水平下降，SREBP-1c蛋白及基因表达下调，且呈现一定的剂量和时间依赖性。 结论 山银花中黄酮类提取物能引起人肝L-02细胞TG含量下降，且这种作用与SREBP-1c蛋白及基因表达下调有一定关系。 |
| 英文摘要: |
| Objective To observe the effect of lavonoid extract from Flos Lonicerae Confusae on the triglyceride accumulation in human L-02 hepatocyte,and to investigate the effect of the protein and mRNA expression of SREBP-1c in this process. Method The effect of the lavonoid extracted from Flos Lonicerae Confusae on lipid-loaded human L-02 hepatocyte,and the lipid droplet density in cells is observed by oil red O staining.Then the lavonoid extracted from Flos Lonicerae Confusae of different density （0,5,10,20 mg/L for 24 h） and different time （0,12,24,48,72 hours of 10 mg/L）had effects on lipid-loaded human L-02 hepatocyte,the level of triglyceride was dected by using 3-glycerol phosphate oxidase method,and the protein and mRNA expression of SREBP-1c was dected by Western blot and real-time quantitative PCR. Results Flavonoid extract from Lonicerae Confusae had effects onipid-loaded human L-02 hepatocyte,the lipid droplet density decreases in cells,the level of triglyceride decreased,the protein and mRNA expression of SREBP-1c were down-regulated,in a dose and time-dependent manner. Conclusion The lavonoid extract from Lonicerae Confusae can degradete the level of triglyceride in lipid-loaded human L-02 hepatocyte,and the possible mechanism is related to the down-regulation of SREBP-1c expression. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |