| 王淑芬，董巍蕾，谢晶，何璐，周曦，蔡艳林，刘杰，谢宛玉.miR-26a通过调节MTDH基因表达抑制CAOV3细胞系生长.[J].中南医学科学杂志.,2014,42(6):563-566. |
| miR-26a通过调节MTDH基因表达抑制CAOV3细胞系生长 |
| miR-26a Inhibits Ovarian Cancer Cells Proliferation by Regulating MTDH |
| 投稿时间：2014-04-14 |
| DOI： |
| 中文关键词: miR-26a 卵巢癌 生长增殖 |
| 英文关键词:miR-26a ovarian cancer growth |
| 基金项目: |
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| 摘要点击次数: 982 |
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| 中文摘要: |
| 目的检测miR-26a在卵巢癌组织的表达改变，明确miR-26a调控卵巢癌细胞生长的分子机制。 方法 运用qRT-PCR检测52例卵巢癌及相应癌旁组织中miR-26a的表达改变；将miR-26a mimics转染卵巢癌细胞CAOV3，以MTDH siRNA为阳性对照，采用Western blot检测其对MTDH蛋白表达水平的影响；然后采用MTT法检测高表达miR-26a对CAOV3细胞生长增殖的影响。结果qRT-PCR检测结果显示，miR-26a在52例卵巢癌组织中表达下调；Western blot结果显示，过表达miR-26a或干扰MTDH可抑制MTDH蛋白的表达。MTT检测发现，过表达miR-26a或干扰MTDH可抑制人CAOV3细胞的生长(P<0.05)。 结论miR-26a通过调节MTDH基因的表达而抑制卵巢癌细胞的生长。 |
| 英文摘要: |
| ObjectiveTo explicit the expression and role of miR-26a in ovarian cancer tissues and cells.MethodsqRT-PCR was used to detect the expression of miR-26a in ovarian cancers and normal ovarian tissues.CAOV3 cells were transfected with miR-26a mimics,and MTDH siRNA as for positive control,then Western blotting was performed to detect the expression of MTDH protein.The proliferation ability of CAOV3 cells was evaluated by MTT assy.ResultsmiR-26a was down-regulated in ovarian cancer tissues.Western blot showed that the expressions of MTDH protein was inhibited by restored miR-26a or siRNA MTDH in CAOV3 cells.Overexpression of miR-26a or siRNA MTDH inhibited the proliferation of CAOV3 cells.ConclusionmiR-26a suppresses cell proliferation by regulating MTDH in ovarian cancer. |
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