| 谢金龙，罗招阳，袁恩健，陈永春，左建宏.Bcl-2与Ki-67蛋白在喉鳞癌中的表达检测及临床相关性分析.[J].中南医学科学杂志.,2014,42(6):549-553. |
| Bcl-2与Ki-67蛋白在喉鳞癌中的表达检测及临床相关性分析 |
| Expression and Analysis of Correlation of Bcl-2 andKi-67 in Laryngeal Squamous Cell Carcinoma |
| 投稿时间：2014-04-01 |
| DOI： |
| 中文关键词: 喉鳞状细胞癌 Bcl-2 Ki-67 增殖 侵袭转移 |
| 英文关键词:Laryngeal squamous cell carcinoma Bcl-2 Ki-67 proliferation invasion and metastasis |
| 基金项目:国家自然科学基金（81272960），湖南省科技厅项目（2013WK2010），湖南省卫生厅项目（B2012-047）和湖南省教育厅项目（12C0343）. |
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| 中文摘要: |
| 目的检测喉鳞状细胞癌中Bcl-2蛋白和Ki-67蛋白的表达水平并分析两者的相关性。方法收集54例喉鳞癌组织和42例正常喉粘膜组织的样本，采用SP免疫组化法观察石蜡切片中Bcl-2、Ki-67蛋白的表达，并结合临床相关病理因素分析在喉鳞状细胞癌中两者的相关性。结果在喉鳞癌组织中，Bcl-2蛋白高表达，阳性表达率70.37%（38/54），正常的喉粘膜组织中Bcl-2蛋白阳性微弱表达或不表达，阳性率9.5%（4/42）（P<0.05）；Bcl-2蛋白表达水平与临床分期、淋巴结转移有明显相关性：在晚期（Ⅲ～Ⅳ期）的表达高于早期（Ⅰ～Ⅱ期）（P<0.05），而其在不同年龄、性别中的表达，差异无统计学意义（P>0.05）。Ki-67蛋白在喉鳞状细胞癌中的表达为81.48%（44/54），在正常组织中表达明显下调仅为16.67%（7/42）；在喉鳞癌中Bcl-2蛋白与Ki-67蛋白的表达呈正相关（rs=0.317，P<0.05）。结论与正常组织比较，在喉鳞癌中Bcl-2蛋白与Ki-67蛋白表达明显上调，并呈正相关，可能两者在喉鳞癌癌变中发挥了协同作用。 |
| 英文摘要: |
| ObjectiveThe expression of Bcl-2 and Ki-67 was detected in human laryngeal squamous cell carcinoma (LSCC) and its correlation was analyzed.MethodsThe immunohistochemical method SP was used to analyze the expression and clinical significance of Bcl-2 and Ki-67 in 54 cases of LSCC and 42 cases of the normal laryngeal mucosa as control and the correlation between the two groups.ResultsIn laryngeal squamous carcinoma,the Bcl-2 protein positive overexpression rate was 70.37% (38/54),while it was weak or had no expression in the normal laryngeal mucosa (P<0.05).The Bcl-2 protein expression level was obviously correlated with clinical stages and lymph node metastasis.Moreover,overexpression of Bcl-2 was observed more frequently in LSCC with late TNM stsges (Ⅲ-Ⅳ) (P<0.05).There was no significant difference in the positive expression of Bcl-2 among gender,age and pathological type (P>0.05).Nuclear antigen Ki-67 was expressed in 81.48% (44/54) of patients with LSCC.The expression in normal laryngeal mucosa was significantly lower only for 16.67%(7/42)(P<0.05).The expression of Ki-67 in LSCC was positively related to the expressions of Bcl-2 (rs=0.317,P<0.05).CouclusionsCompared with laryngeal normal tissue,Bcl-2 and Ki-67 protein expression significantly raised in laryngeal squamous cell carcinoma and were positively correlated with each other,which could both play a collaborative role in the carcinogenesis of laryngeal squamous cell carcinoma. |
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