| 曾海燕，方勇，曾高峰.丹红注射液对脂多糖诱导THP-1巨噬细胞促炎因子分泌的影响及机制.[J].中南医学科学杂志.,2014,42(5):443-446. |
| 丹红注射液对脂多糖诱导THP-1巨噬细胞促炎因子分泌的影响及机制 |
| Effect and Mechanisms of Danhong Injection on Proinflammatory Cytokines Release from THP-1 Macrophages Induced by Lipopolysaccharides |
| 投稿时间：2014-03-20 |
| DOI： |
| 中文关键词: 丹红注射液 促炎因子 核因子κB Toll样受体4 动脉粥样硬化 |
| 英文关键词:Danhong injection proinflammatory cytokines nuclear factor-κB Toll-like receptor 4 atherosclerosis |
| 基金项目:衡阳市科技局项目资助（2103K159）. |
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| 中文摘要: |
| 目的观察丹红注射液对脂多糖（LPS）诱导的THP-1巨噬细胞促炎因子分泌的影响，并探讨其机制。方法160 nmol/L佛波酯孵育THP-1巨噬细胞24 h后分为对照组、脂多糖组、丹红组和脂多糖+丹红组，酶联免疫吸附法检测培养液中促炎因子的含量，Western blot检测核因子κB(NF-κB)、p65和Toll样受体4（TLR4）的蛋白表达水平。结果丹红注射液明显抑制LPS诱导的巨噬细胞肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)释放，下调核因子NF-κB和TLR4的表达。结论丹红注射液抑制脂多糖诱导的炎症反应，可能与其抑制TLR4和NF-κB的表达有关。 |
| 英文摘要: |
| ObjectiveThis study was to investigate the effect of Danhong injection on proinflammatory cytokine release from lipopolysaccharide (LPS)-induced THP-1 macrophages.MethodsHuman THP-1 macrophages were induced using phorbol-12-myristate acetate (PMA，160 nmol/L) for 24 h，then cells were divided into four groups:control group，LPS group，Danhong group and LPS＋Danhong group.Secretion of proinflammatory cytokines，including tumor necrosis factor-α (TNF-α)，interleukin-6 (IL-6) and interleukin-1β (IL-1β) was performed by enzyme-linked immunosorbent assay (ELISA).The protein expression of nuclear factor-κB (NF-κB) p65 and Toll-like receptor 4 (TLR4) were examined by Western blot.ResultsDanhong injection inhibited the release of TNF-α，IL-6 and IL-1β induced by LPS，repressed the translocation of NF-κB to the nucleus and TLR4 expression.ConclusionsDanhong injection inhibited the release of inflammatory cytokine that induced by LPS，and the mechanisms may be related to the TLR4 and NF-κB expressions. |
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