| 魏付桥，申清香，刘昌化，罗康宁，谢文彪，胡军.PEITC通过抑制结肠癌细胞PI3K/NF-κB活性而抑制MMP-9表达.[J].中南医学科学杂志.,2014,42(4):351-354. |
| PEITC通过抑制结肠癌细胞PI3K/NF-κB活性而抑制MMP-9表达 |
| Phenethyl Isothiocyanate Inhibits PI3K/NF-κB to Down-regulate MMP-9 Expression in Human Colon Cancer Cells |
| 投稿时间：2013-11-19 |
| DOI： |
| 中文关键词: 异硫氰酸苯乙酯 SW480细胞 基质金属蛋白酶9 PI3K/Akt 核转录因子κB |
| 英文关键词:PEITC SW480 cells MMP-9 PI3K/AKT NF-κB |
| 基金项目:湖南省科技计划项目（2013SK3120）. |
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| 中文摘要: |
| 目的观察异硫氰酸苯乙酯（PEITC）对结肠癌细胞基质金属蛋白酶9（MMP-9）表达的影响及其分子机制。方法体外培养结肠癌细胞系SW480，分别用10、30、50 μmol/L PEITC作用24 h，划痕愈合实验和侵袭实验分别检测PEITC对SW480细胞迁移与侵袭的影响。RT-PCR检测MMP-9 mRNA的表达；Western blot检测MMP-9蛋白表达和蛋白激酶B（Akt）磷酸化。荧光素酶报告基因分析核转录因子κB（NF-κB）和MMP-9的活性。 |
| 英文摘要: |
| ObjectiveTo investigate the effect and molecular mechanism of PEITC on matrix metalloproteinase-9 (MMP-9) expression in human colon cancer SW480 cells.MethodsHuman colon cancer SW480 cell line was cultured in vitro,and treated with 10,30,50μmol/L PEITC for 24h.Cell viability,cell migration and invasion was analyzed by MTT assay,scratch healing assay and transwell membrane assay,respectably.Expression of MMP-9 mRNA was analyzed by RT-PCR.Protein expression and phosphorylation of Akt were detected by Western blot.Activity of NF-κB and MMP-9 were analyzed by luciferase reporter assay.ResultsPEITC reduced SW480 cells invasion and migration without affecting the viability of cells,as demonstrated by scratch healing and transwell membrane assay.Furthermore,Western blot and RT-PCR showed PEITC markedly inhibited MMP-9 activity and expression at both protein and mRNA levels.PEITC attenuated Akt phosphorylation and activity of NF-κB.Moreover,LY294002,an inhibitor of PI3K,significantly inhibited NF-κB transcriptional activity and MMP-9 expression.In addition,different concentration of PEITC could inhibit the promotion activity of MMP-9.Conclusion |
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