| 刘哲伟，苏开新，陈军，姚建，李辉，王海华，赵鑫.热应激预处理影响鼠背轴型皮瓣HSP70、HSF1表达的实验研究.[J].中南医学科学杂志.,2014,42(3):255-257. |
| 热应激预处理影响鼠背轴型皮瓣HSP70、HSF1表达的实验研究 |
| Study of Heat Stress Pretreatment Effect on Expressions of HSP70，HSF1 in Axial Flap of Rats |
| 投稿时间：2013-12-14 |
| DOI： |
| 中文关键词: 热应激预处理 轴型皮瓣 热休克蛋白70 热休克因子1 |
| 英文关键词:heat stress pretreatment axial flap HSP70 HSF1 |
| 基金项目: |
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| 中文摘要: |
| 目的探讨热应激预处理（HSP）对缺血皮瓣组织的保护作用及其与皮瓣中热休克蛋白70（HSP70）和热休克因子1（HSF1）的关系。方法将96只雄性Sprague Dawley 大鼠随机分为缺血组与实验组（HSP+缺血组）。缺血组：皮瓣模型制备后,阻断皮瓣蒂部第1 h、2 h、4 h、6 h、8 h、10 h共6个时间点，每时间点后观测皮瓣存活面积、HSP70、 HSF1的变化，并进行统计学分析；实验组（HSP+缺血组）：先予以加热预处理，之后与缺血组相同。结果单纯缺血组皮瓣成活率下降率较实验组（HSP+缺血组）明显，差异有显著性(P<0.05),热应激预处理各组皮瓣的HSP70 、HSF1合成量与皮瓣存活率呈正相关(r=0.7577，P<0.01)。结论热应激预处理能够改善皮瓣组织的存活率，HSP70、HSF1在热应激预处理改善皮瓣存活率的保护作用中发挥着重要的作用。 |
| 英文摘要: |
| ObjectiveThis study was to reveal the continuous changes of HSP70 and HSF1 in the delayed and protection of heat stress pretreatment on ischemic preconditioning axial flap in rats.MethodsAnimals were divided into two groups randomly:HSP+IPC group，ischemic pretreatment group（IPC）.In IPC group,rats skin flaps received ischemic pretreatment for 1 h，2 h，4 h，6 h，8 h，10 h,and the survival rate of the skin flaps,the contents of HSP70 and HSF1 were examined at the 6 time points.HSP+IPC group was given heat stress pretreatment and then the same treatment as IPC group.ResultsThe survival rates of IPC descends more significantly than that of HSR+IPC.The survival rates of skin flaps have positive correlation with the contents of HSP70 and HSF1(r=0.7577，P<0.01).ConclusionsHeat stress pretreatment can improve the survival rate of ischemia flap and the mechanism of the protection can be related to the function of HSP70 and HSF1. |
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