| 欧阳德亮，胡杨，王又保，黄明明，谢荣兵.穿心莲内酯对胃癌AGS细胞增殖侵袭的影响.[J].中南医学科学杂志.,2014,42(3):246-250. |
| 穿心莲内酯对胃癌AGS细胞增殖侵袭的影响 |
| Effect of Andrographolide on Human AGS GastricCancer Cells Proliferation and Invasion |
| 投稿时间：2013-08-19 |
| DOI： |
| 中文关键词: 穿心莲内酯 胃癌细胞 Na+-K+-ATP酶 增殖 侵袭 |
| 英文关键词:Andrographolide gastric cancer cells Na+-K+-ATPase proliferation invasion |
| 基金项目:衡阳市2012年科学技术发展项目（2012KJ18）. |
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| 中文摘要: |
| 目的观察穿心莲内酯（AD）对胃癌细胞增殖侵袭以及Na+-K+-ATP酶活性的影响。方法体外培养胃癌细胞系AGS，分别用1.0、3.0、5.0 μmol/L穿心莲内酯作用24～72 h，无机磷法检测AGS细胞Na+-K+-ATP酶活性改变情况；MTT和ELISA分析AGS细胞的增殖与断裂DNA含量；同时采用ELISA检测血管内皮生长因子（VEGF）和转化生长因子β1（TGF-β1）的水平；荧光共振能量转移法检测基质金属蛋白酶9（MMP-9）的活性；小室侵袭实验检测细胞侵袭。结果1.0～5.0 μmol/L穿心莲内酯能显著降低Na+-K+-ATP酶活性，并能在此浓度下抑制AGS细胞生长增殖以及增加断裂DNA水平。同时，穿心莲内酯也能减少VEGF和TGF-β1的水平（P<0.05），降低MMP-9酶的活性，并能有效减少癌细胞的侵袭（P<0.05）。结论穿心莲内酯是一种有效的抗胃癌药物，它可能具有阻碍胃癌细胞侵袭的能力。 |
| 英文摘要: |
| ObjectiveTo observe the effect of Andrographolide on the proliferation and invasion of human gastric cancer cells and explore its potential molecular mechanism.MethodsHuman gastric cancer cell line AGS was cultured in vitro,and was incubated with 1.0,3.0 and 5.0μmol/L andrographolide.After treatment,enzymic activity of Na+-K+-ATPase was determined by norganic phosphorus assay.Proliferation of AGS cells and the DNA fragments were detected by MTT assay and ELISA,respectively.Production of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β) were detected by ELISA.Activity of Matrix metalloproteinase-9 (MMP-9) was analyzed by a fluorescence resonance energy transfer kits.Chamber invasion assay was used to study the cell invasion.Results1.0～5.0 μmol/L of andrographolide significantly reduced the enzymic activity of Na+-K+-ATPase,suppressed the AGS cells growth and induced the formation of DNA fragment,as demonstrated by MTT and ELISA.Results also showed that andrographolide could also decrease the VEGF and TGF-β1 level,and decrease the enzymic activity of MMP-9.In addition,andrographolide could also reduce AGS cells invasion.ConclusionAndrographolide is a potential anti-cancer drug,and it could inhibit gastric cancer cells proliferation and invasion. |
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