| 孙秀才，施霞，曾辉，孟娟，盛艳华，冯建章，.姜黄素通过抑制ERK1/2磷酸化减弱ET-1诱导的大鼠VSMCs增殖.[J].中南医学科学杂志.,2014,42(2):144-147. |
| 姜黄素通过抑制ERK1/2磷酸化减弱ET-1诱导的大鼠VSMCs增殖 |
| Effect of Curcumin on Proliferation of Rat VascularSmooth Muscle Cells Induced by Endothelin-1 and Expression of Phosphorylated ERK1/2 |
| 投稿时间：2013-12-12 |
| DOI： |
| 中文关键词: 姜黄素 内皮素-1 血管平滑肌细胞 p44/42丝裂原活化蛋白激酶 |
| 英文关键词:curcumin endothelin-1 vascular smooth muscle cell p44/42 mitogen-activated protein kinase |
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| 中文摘要: |
| 目的观察姜黄素（curcumin）对内皮素-1（ET-1）诱导培养的离体大鼠血管平滑肌细胞(VSMCs)增殖及p44/42丝裂原活化蛋白激酶（p44/42 MAPK,ERK1/2）表达的影响。方法组织贴块法培养大鼠胸主动脉VSMCs。分别以ET-1 10-8 mol/L，ET-1 10-8 mol/L联合浓度为10-6 mol/L、10-5 mol/L、10-4 mol/L的姜黄素作用于体外培养的VSMCs 24 h，采用细胞计数检测VSMCs的增殖，3H-胸腺密啶掺入（3H-TdR）法测定VSMCs的DNA合成，western bolt法检测VSMCs磷酸化ERK1/2的表达。结果与对照组比较，ET-1能显著刺激VSMCs增殖，姜黄素能显著抑制ET-1诱导的VSMCs增殖，且呈剂量依赖关系；ET-1能显著刺激VSMCs磷酸化ERK1/2的表达，该作用可被姜黄素所抑制。结论姜黄素能抑制ET-1刺激的VSMCs的增殖，其作用可能与抑制磷酸化ERK1/2的表达相关。 |
| 英文摘要: |
| ObjectiveTo study effect of curcumin on proliferation of rat vascular smooth muscle cells（VSMCs）induced by endothelin-1(ET-1) and expression of phosphorylated p44/42 mitogen-activated protein kinase (p44/42 MAPK,ERK1/2).MethodsRat VSMCs cultured in vitro were exposed for 24 h to 10-8 mol/L ET-1,10-8 mol/L ET-1 plus 10-6 mol/L,10-5 mol/L,10-4 mol/L curcumin repectively.The effect of curcumin on VSMCs growth and DNA synthesis were investigated by cell counting,3H-thymidine incorporation,the expression of phosphorylated ERK1/2(p-ERK1/2) were determined by western blotting.ResultsCompaered with control group,VSMCs exposed to ET-1 showed significantly enhanced proliferation (P<0.05),curcumin could inhibit proliferation of VSMCs induced by ET-1(P<0.05),there are dose dependent inhibitory effects in different concentration of curcumin.ET-1 treatment also increased significantly the expression of p-ERK1/2,curcumin could decrease the effection (P<0.05).ConclusionCurcumin can inhibit the proliferation of rat VSMCs and the expression of p-ERK1/2 induced by ET-1. |
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