| 蔡晓健，丁涛，段友军，黄振国，刘志文，王豪，刘未斌，许韩峰1.前列腺癌膜联蛋白Ⅱ微卫星不稳定的荧光复合扩增研究.[J].中南医学科学杂志.,2014,42(1):47-51. |
| 前列腺癌膜联蛋白Ⅱ微卫星不稳定的荧光复合扩增研究 |
| The Study of Fluorescent Multiplex PCR Amplification toDetect Annexin Ⅱ Microsatellite Instability in Prostate Cancer |
| 投稿时间：2013-10-10 |
| DOI： |
| 中文关键词: 前列腺癌 膜联蛋白Ⅱ 荧光复合扩增 微卫星 |
| 英文关键词:Prostate cancer Annexin Ⅱ Fluorescence multiplex amplification Microsatellite |
| 基金项目:湖南省科技厅项目课题资助（编号2011SK3189）. |
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| 中文摘要: |
| 目的探讨膜联蛋白Ⅱ微卫星的不稳定对前列腺癌早期诊断的作用。方法使用荧光复合扩增的方法对16例前列腺癌组织、16例良性前列腺增生组织及16例正常人血样中的膜联蛋白Ⅱ的三个基因座进行扩增，检测PCR产物，分析其差异与疾病间的关系。结果膜联蛋白Ⅱ的D15S1185，D15S1292两个正常基因座在前列腺癌组织、良性前列腺增生组织以及血液样本中扩增大小无差别；在AN2-di-repeat-NH的二核苷酸重复序列微卫星基因座区域表现差异，以扩增大小134 bp为分界线，前列腺癌组织与正常血液样本存在显著性差异（χ2=9.087,df=1,P=0.003），前列腺癌组织与良性前列腺增生组织之间显著性不明显（χ2=1.997,df=1,P=0.158），良性前列腺增生组织与正常血液样本之间有显著性差异趋势（χ2=3.405,df=1,P=0.065）。结论膜联蛋白Ⅱ二核苷酸微卫星基因座AN2-di-repeat-NH的多态性有可能成为早期前列腺癌的预警指标。 |
| 英文摘要: |
| ObjectiveTo study the effect of annexin Ⅱ microsatellite instability on diagnosing the early prostate cancer.MethodFluorescent multiplex PCR was used to amplify annexin Ⅱ three loci in 16 prostate cancer tissues,16 benign prostatic hyperplasia tissues and 16 normal blood samples.Then,the 48 PCR products were collected and the differences and relationship between them were analyzed.ResultAnnexin Ⅱ D15S1185,D15S1292 loci have the same size PCR product in all the samples;AN2-di-repeat-NH dinucleotide repeats locus performs differences in size of 134 bp.Significant differences exist between prostate cancer tissues and normal blood samples(χ2=9.087,DF=1,P=0.003).Prostate cancer tissues and benign prostatic hyperplasia tissues didnt have obviously significant difference.(χ2=1.997,DF=1,P=0.158).Benign prostatic hyperplasia tissues and normal blood samples have significant trend （χ2=3.405,df=1,P=0.065）.ConclusionThe polymorphisms of a novel AN2-di-repeat-NH dinucleotide repeats locus may be a warning indicator of early prostate cancer. |
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