| ObjectiveTo discuss the diagnostic effective indicators of anti-nuclear antibody(ANA) fluorescence patterns and extractable nuclear antibody(ENA) for the patients with lupus nephritis (LN).MethodsWe enrolled 158 systemic lupus (SLE) patients,of whom 80 were diagnosed with LN,the other 78 were not accompanied with LN,104 people without SLE were regarded as control group.Autoantibodies were measured and analyzed in 158 SLE patients and control group.The levels of ANA and anti-double stranded DNA(anti-dsDNA) antibody were measured by indirect immunofluorometric assay,and other 11 anti-ENA antibody were determined by linearity immunoblotting assay.RusultsAt the study entry,The positive rate of ANA（>1∶100）in LN patients were 100%（80/80）,42.5%（34/80） LN patients present homogeneous pattern in the sero,which were significantly more than that in SLE patients (21.79%,17/78);While granular pattern in SLE group(73.08%,57/78） was obviously higher than that of LN group(53.75%,43/80）,P<0.05.In this article,we demonstrated that anti-Hi,anti-Nud,anti-dsDNA antibody were cardinal target antibodies of homogeneous pattern,pearsonχ2 were 8.26,23.05,26.39,individually,P<0.05.Through manifestation by chi-spuare test,with regard to LN patients,only prevalence for anti-dsDNA antibody (55.00% vs.23.08%,P=<0.05)in patients with LN were significantly higher than that in patients without LN.ConclusionsThe main fluorescence patterns were homogeneous pattern in sero of SLE accompanied with lupus nephritis.And there were high relationship between ANA homogeneous patterns and anti-dsDNA antibody.As a result,detection of the homogeneous pattern and anti-dsDNA antibody was the effective indicator of diagnosis for LN. |