| 胡杨，吴清，刘朝霞，傅念，谢妮，阳学风.埃索美拉唑对幽门螺杆菌诱导胃上皮细胞表达细胞因子的影响.[J].中南医学科学杂志.,2013,41(5):449-452. |
| 埃索美拉唑对幽门螺杆菌诱导胃上皮细胞表达细胞因子的影响 |
| Effect and Molecular Mechanism of Esomeprazole on Helicobacterpylori-induced Cytokines Production in Gastric Epithelial Cells |
| 投稿时间：2013-06-03 |
| DOI： |
| 中文关键词: 血红素氧合酶-1 埃索美拉唑 幽门螺杆菌 胃上皮细胞 白细胞介素 |
| 英文关键词:heme oxygenase-1 esomeprazole helicobacter pylori gastric epithelial cells IL |
| 基金项目:衡阳市科技局科学技术发展项目（2012KJ18）. |
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| 中文摘要: |
| 目的探讨埃索美拉唑对幽门螺杆菌（Hp）感染的胃上皮细胞的体外抗炎效应。方法体外培养胃上皮细胞系AGS，加入不同浓度埃索美拉唑和培养的Hp感染8或24 h。Western blot检测和实时定量PCR分别检测AGS细胞中红素氧合酶1（HO-1）蛋白和mRNA表达情况；ELISA检测白细胞介素-1β（IL-1β）和IL-8的产生。同时采用RNA干扰HO-1表达后，观察其对Hp诱导IL-1β和IL-8产生的影响。结果埃索美拉唑孵育8 h后，能显著抑制Hp诱导的IL-1β和IL-8表达。同时，埃索美拉唑能在转录翻译水平分别调控HO-1 mRNA和蛋白表达。HO-1 siRNA沉默HO-1表达后，与Hp组相比，IL-1β和IL-6分泌进一步增高。结论埃索美拉唑可能通过上调HO-1的表达而发挥对胃上皮细胞的抗炎作用。 |
| 英文摘要: |
| ObjectiveTo investigate the anti-inflammatory effects of Esomeprazole in H.pylori-infected gastric epithelial cells in vitro.MethodsGastric epithelial cell line AGS was cultured in vitro,and was incubated with Esomeprazole and H.pylori for 8 or 24h.Expression of Heme oxygenase-1 mRNA and protein was detected by Western blot and real-time PCR,respectively;production of IL-6 and IL-8 were detected by ELISA.RNA interference was used to silent HO-1 expression,and H.pylori-induced production of IL-1β and IL-8 were detected.ResultsAfter incubated for 8 h,esomeprazole could significantly inhibit H.pylori induced IL-1β and IL-8 production.And esomeprazole could also induce AGS cells expression of HO-1 mRNA at the transcriptional and translational level.Furthermore,silencing of HO-1 expression could increase AGS cell secretion of IL-1β and IL-8,as compared with that in H.pylori group.ConclusionHO-1 is a target of esomeprazole in gastric epithelial cells.HO-1 induction might account for the gastroprotective effects in vasoprotection. |
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