| 欧琼，张志伟，罗招阳，刘重元，杨科，崔责文，赵强，陈波.EGCG通过降低Notch1和Jagged1表达抑制鼻咽癌干细胞SP18的增殖.[J].中南医学科学杂志.,2013,41(5):440-444. |
| EGCG通过降低Notch1和Jagged1表达抑制鼻咽癌干细胞SP18的增殖 |
| EGCG Decreased the Expression of Notch1 and Jagged1 Proteins andInhibited the Proliferation of Nasopharyngeal Cancer Stem Cell SP18 |
| 投稿时间：2013-05-16 |
| DOI： |
| 中文关键词: EGCG 鼻咽癌干细胞SP18 细胞增殖 Notch1蛋白 Jagged1蛋白 |
| 英文关键词:Epigallocatechin-3-gallate nasopharyngeal cancer stem cell SP18 cellular proliferation Notch1 protein Jagged1 protein |
| 基金项目:湖南省自然科学省市联合基金（12JJ9033）；湖南省自然科学基金（12JJ3102、13JJ3079）；湖南省高校创新平台开放基金（12K094、10K052）；湖南省研究生科研创新项目（CX2010B380）；湖南省教育厅科研项目（11C1112、12C0340）；衡阳市科技局科研项目（2011kj52、2011kj53）. |
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| 中文摘要: |
| 目的探讨表没食子儿茶素没食子酸酯（EGCG）抑制鼻咽癌干细胞SP18增殖的机制。方法采用MTT法检测EGCG抑制SP18细胞增殖的IC50；选用细胞生长曲线、平皿克隆形成实验和软琼脂集落形成实验观察EGCG对鼻咽癌干细胞SP18增殖的影响；采用Western blot检测Notch1和Jagged1蛋白表达。结果EGCG抑制SP18细胞增殖的IC50值为149.02 mg/L；EGCG呈浓度和时间依赖性抑制SP18细胞的增殖（n=3，P<0.05）；EGCG呈浓度依赖性抑制Notch1和Jagged1蛋白的表达。结论EGCG能有效抑制鼻咽癌干细胞SP18的增殖，其可能通过降低Notch1和Jagged1蛋白的表达而发挥作用。 |
| 英文摘要: |
| ObjectiveTo analyze the mechanism of Epigallocatechin-3-gallate (EGCG) inhibiting the proliferation of nasopharyngeal cancer stem cell SP18.Methods50% inhibiting concentration (IC50) of which EGCG inhibited the proliferation of nasopharyngeal cancer stem cell SP18 was detected by MTT method.The proliferation of SP18 cells affected by EGCG was analyzed by the cellular growth curve,colony formation and soft agar formation.Then the expression of Notch1 and Jagged1 protein were respectively detected by Western blot in SP18 cells treated by EGCG.ResultsIC50,of which EGCG inhibted the proliferation of SP18 cells,was 149.02 mg/L.The proliferation of SP18 cells was inhibited with EGCG concentration increasing and treatment time extending (n=3,P＜0.05).The expression of Notch1 and Jagged1 proteins in SP18 cells was obviously deceased with EGCG concentration increased.ConclusionEGCG can inhibit the proliferation of nasopharyngeal cancer stem cell SP18,which probably decreased the expression of Notch1 and Jagged1 proteins to achieve the role. |
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