| 曾勇智，汪煜华，赵战芝.二烯丙基二硫抑制HL-60细胞增殖及对c-myc基因表达调控.[J].中南医学科学杂志.,2013,41(4):347-349. |
| 二烯丙基二硫抑制HL-60细胞增殖及对c-myc基因表达调控 |
| Proliferation Inhibition and c-myc Expression Down-Regulated by Diallyl Disulfide in HL-60 Cells |
| 投稿时间：2013-03-07 |
| DOI： |
| 中文关键词: 二烯丙基二硫 c-myc HL-60细胞 |
| 英文关键词:Diallyl disulfide c-myc HL-60 cells |
| 基金项目:湖南省卫生厅课题（B2010-041）. |
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| 中文摘要: |
| 目的研究二烯丙基二硫（DADS）对人髓系白血病细胞株HL-60细胞增殖及对c-myc基因表达的影响。方法采用MTT和细胞记数法分析DADS对细胞生长的影响、蛋白印迹法检测细胞内c-myc蛋白表达水平。结果 DADS能抑制HL-60细胞增殖，并呈剂量和时间依赖性（P<0.05），DADS（20 μmol/L）作用12 h后能下调c-myc蛋白的表达水平。结论DADS抑制HL-60细胞增殖，其机制可能与下调c-myc表达水平密切相关。 |
| 英文摘要: |
| ObjectiveTo investigate the effects of DADS on proliferation inhibition and on c-myc protein expression in human myeloid leukemia cell line HL-60 cells.MethodsHL-60 cells were exposed to DADS at different dosages.Growth inhibition was detected by MTT assay and cell growth.The expression of c-myc was measured by Western blotting.ResultsAfter treatment with DADS, the growth of HL-60 cells were suppressed in a concentration-dependent and time-dependent manner（P<0.05）.Incubation of HL-60 cells with DADS（20 μmol/L）for 12 h could decrease the expression of c-myc.ConclusionDADS could inhibit HL-60 cells proliferation，its mechanism may be closely related with adjusting expression level of the c-myc． |
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