| 肖新华，李菡，杨娇娇，冉莉，杨靖，刘江华，文格波.锌alpha2糖蛋白在小鼠NAFLD发展中的动态变化.[J].中南医学科学杂志.,2013,41(4):336-310. |
| 锌alpha2糖蛋白在小鼠NAFLD发展中的动态变化 |
| Dynamic Observation of Zinc alpha2 Glycoprotein in Mice of Non-alcoholic Fatty Liver Disease |
| 投稿时间：2013-01-18 |
| DOI： |
| 中文关键词: 非酒精性脂肪性肝病 锌alpha2糖蛋白 高脂饮食 肥胖 小鼠 |
| 英文关键词:nonalcoholic fatty liver disease Zinc alpha2 glycoprotein high fat diet obesity mice |
| 基金项目:国家自然科学基金资助项目（81270925），湖南省自然科学基金重点项目（12JJ2050）. |
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| 中文摘要: |
| 目的建立高脂饲料喂养的非酒精性脂肪性肝病(NAFLD)小鼠模型，动态观察锌alpha2糖蛋白（ZAG）的表达变化，初步探讨ZAG与NAFLD的关系。方法将48只8周龄C57BL/6小鼠随机分成高脂饮食组(HFD组)和标准饮食组(SD组)，分别给予高脂饲料和标准饲料饲养4周、8周、12周和16周。HE染色分析肝脏组织病理学改变。酶联免疫吸附法（ELISA）检测肝脏甘油三酯（TG）含量、血清ZAG以及炎症因子水平。荧光实时定量聚合酶链反应（PCR）检测肝脏ZAG的mRNA表达。 结果4周开始，HFD组小鼠血清肿瘤坏死因子α（TNF-α）较SD组小鼠明显升高(P<0.05)，且随非酒精性脂肪性肝病的发展而持续增加。8周起HFD组小鼠白细胞介素6（IL-6）、IL-8以及IL-1β水平逐渐升高(P<0.05)。4周起，HFD组小鼠肝脏ZAG mRNA表达逐渐下降(P<0.05)，而血清ZAG浓度从8周开始明显降低(P<0.05)。 结论肝脏ZAG表达水平降低可能参与NAFLD的发生。 |
| 英文摘要: |
| ObjectiveTo establish a nonalcoholic fatty liver disease model induced by high fat diet and observe the dynamic changes of Zinc alpha2 glycoprotein. MethodsAll of 48 C57BL/6 mice were randomly divided into high fat diet (HFD) group and standard diet（SD）group. The serum IL-6, IL-8, TNF-α,IL-1β and ZAG were assayed by ELISA kit at the 4th, 8th, 12th and 16th week. Hepatic triglyceride content was detected by ELISA kit. The liver tissues of mice were stained by HE. The mRNA expression of ZAG in liver was detected by real-time PCR.ResultsCompared with SD group, the level of serum TNF-α was significantly increased in HFD group from the 4th week (P<0.05). From the 8th week, the weight gain, liver index, hepatic triglyceride content, IL-6，IL-8 and IL-1β were increased gradually(P<0.05). The mRNA level of ZAG in liver of mice induced by HFD was gradually decreased from the 4th week (P<0.05). From the 8th week, the serum ZAG in HFD group was decreased compared to SD group (P<0.05).ConclusionThe mouse model of NAFLD could be successfully established with high fat diet. The low expression level of ZAG in liver could be associated with the development of NAFLD. |
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