| 刘世敏，李解方.HSP27在前列腺癌组织中的表达及其临床意义.[J].中南医学科学杂志.,2013,41(3):247-252. |
| HSP27在前列腺癌组织中的表达及其临床意义 |
| The Expression of HSP27 in Plrostate Carcinoma and ItsImplication in Clinics |
| 投稿时间：2013-03-27 |
| DOI： |
| 中文关键词: HSP27 前列腺癌 分化 转移 |
| 英文关键词:HSP27 Prostate Cancer Gleason score metastasis PSA. |
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| 中文摘要: |
| 目的研究热休克蛋白27（HSP27）与前列腺癌恶性程度的相关性。方法采用免疫组化检测75例前列腺癌和30例前列腺增生组织中HSP27的表达，分析HSP27表达与前列腺癌的Gleason评分（分化程度）、转移、临床分期和前列腺癌患者血清中PSA水平之间的关系；Western blot检测HSP27在不同转移潜能前列腺癌细胞中的表达水平；siRNA降低前列腺癌细胞中HSP27的表达，Transwell检测HSP27表达下调后前列腺癌细胞侵袭转移能力的变化。结果30例良性前列腺增生组织中HSP27阳性表达率为23.3%，75例前列腺癌组织中HSP27阳性表达率为65.3%，两者之间有显著性差异（P<0.05）；在Gleason分数分别为2～4分、5～7分和8～10分的三组不同分化程度的前列腺癌组织中，HSP27表达阳性率分别为40.0%、68.8%和94.4%，三者之间HSP27表达有显著性差异（P<0.05）；在血清PSA水平分别为＜10 ng/mL、10～20 ng/mL和＞20 ng/mL的三组前列腺癌患者中，HSP27阳性表达率分别为25.0%、59.5%和92.3%，三个不同PSA水平的组别之间HSP27阳性表达率有显著性差异（P<0.05）;HSP27表达水平与前列腺癌TNM分期有关 (P＜0.05)；与低转移潜能前列腺癌细胞系LNCaP相比，在高转移潜能前列腺癌细胞系C4-2B中HSP27表达水平明显上调，两者之间有显著性差异(P<0.01)；Transwell侵袭实验显示，下调HSP27表达能低前列腺癌细胞的体外侵袭能力（P<0.05）。结论HSP27表达与前列腺癌的Gleason评分、转移、临床分期和血清中PSA水平相关，HSP27可作为判断前列腺癌恶性程度和靶向治疗的分子标记物。 |
| 英文摘要: |
| ObjectiveTo investigate relationship between the expression of Heat Shock Protein 27（HSP27） and Prostate Cancer (PC).MethodsExpression of HSP27 were determined by immunohistochemical method in 30 cases of BPH and 75 case of PC.The correlation among the expression of HSP27 and clinicopathologic factors were evaluated through retrospective research.The relationship among the expression of HSP27 and Gleason score,metastasis,TNM stage and serum level of PSA in PC were analysized by SPSS17.0 software.The level of HSP27 were detected by western bloting in prostatic carcinoma cell lines with different metastatic potency.After performed siRNA to PC cell line,the expression of HSP27 was down-regulated and the Transwell test were performed to detected the invasive and metastatic capability.ResultsThe positive ratio of HSP27 expression were 65.3% in 75 cases of PC,and 23.3% in 30 cases of BPH.And there were significantly difference between PC and BPH (P<0.05).The positive ratio of HSP27 expression were 40.0%、68.8% and 94.4% in 75 patiens of PC with Gleason 2～4 score,5～7 score,8～10 score,respectively.And there were significantly difference among them (P<0.05).The positive ratio of HSP27 expression were 25.0%,59.5%,92.3% in PC patients with PSA＜10 ng/mL,10～20 ng/mL,＞20 ng/mL,respectively.And there were significantly difference among the three groups with differential level of PSA in PC (P<0.05).There was relationship among the expression of HSP27 and TNM stages (P<0.05).The expression level of HSP27 were higher in C4-2B cell lines with high metastatic ability than that of LNCaP cell lines with lowly metastatic ability,there were significantly difference between them(P<0.01).The Transwell test showed that down-regulation of HSP27 could result into the decreasing of in vitro invasive ability in PC cells (P<0.05）.ConclusionThere were relationship among the expression of HSP27 and Gleason score,metastasis,TNM stage and serum level of PSA in PC and HSP27 could serve as a biomarker for decision of malignant degree and targeted therapy in PC. |
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