| 唐彩霞，张树友，何英新.外源性硫化氢通过ATP敏感钾通道诱导人子宫肌瘤细胞凋亡.[J].中南医学科学杂志.,2013,41(3):238-242. |
| 外源性硫化氢通过ATP敏感钾通道诱导人子宫肌瘤细胞凋亡 |
| Extrogenous Hydrogen Sulfide Induces the Apoptosis Through ATP-sensitive K+ Channels in Human Uterine Leiomyoma Cells |
| 投稿时间：2013-01-14 |
| DOI： |
| 中文关键词: 硫化氢 子宫肌瘤细胞 凋亡 ATP敏感钾通道 p53 bcl-2 |
| 英文关键词:hydrogen sulfide uterine leiomyoma cells apoptosis aTP-sensitive K+ channels p53 bcl-2. |
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| 中文摘要: |
| 目的探讨ATP敏感钾通道（KATP）在外源性硫化氢（H2S）诱导人子宫肌瘤细胞凋亡中的作用。方法用硫氢化钠(NaHS)作为H2S的供体处理原代培养的人子宫肌瘤细胞，KATP通道抑制剂格列本脲(Gly)预处理细胞。实验分为对照组、不同浓度NaHS组、ATP敏感性钾通道（KATP）抑制剂组和Gly + NaHS组。用流式细胞术检测细胞凋亡率，采用实时定量PCR和Western blot分别检测p53和bcl-2 mRNA和蛋白的表达。结果与对照组比较，NaHS（10-4 和10-3 mol/L）处理人子宫肌瘤细胞24和48 h后以剂量和时间依赖的方式增加了细胞的凋亡率（均P<0.05）。与对照组比较，NaHS（10-4 mol/L）处理人子宫肌瘤细胞24 h后p53的表达显著增加（P＜0.05），而bcl-2表达显著降低（P＜0.05）。Gly没有影响人子宫肌瘤细胞的凋亡率、p53和bcl-2的表达，但部分地拮抗NaHS的致凋亡作用以及NaHS诱导的子宫肌瘤细胞中p53表达的上调和bcl-2表达的下调（均P<0.05）。结论外源性H2S诱导子宫肌瘤细胞凋亡，其机制与H2S开放KATP通道有关。 |
| 英文摘要: |
| ObjectiveTo explore the role of ATP-sensitive K+ channels in the apoptosis induced by extrogenous hydrogen sulfide (H2S) in human uterine leiomyoma cells.MethodsThe human uterine leiomyoma cells were treated with a H2S donor sodium bisulfide (NaHS).The inhibitor of ATP-sensitive K+ channels glybenclamide (Gly) was used.The cells were divided into control,the different concentration NaHS,Gly and NaHS + Gly groups.The rates of apoptosis of cells were measured by flow cytometry.The mRNA and protein expressions of p53 and bcl-2 in human leimyoma cells were measured by Real-time PCR and Western-Blot.ResultsCompared with the control,the rates of apoptosis were significantly increased in human uterine leiomyoma cells by treatment with NaHS (10-4 and 10-3 mol/L) for 24 and 48 h in a dose-dependent and time-dependent manner.Compared with the control,the expression of p53 was significantly up-regulated and expression of bcl-2 was significantly down-regulated by treatment with NaHS (10-4 mol/L) for 24 h.Gly did not affect the rates of apoptosis,expression of p53 and bcl-2 in human uterine leiomyoma cells.Gly partly blocked the effect of H2S which induced the apoptosis,up-regulated the expression of p53 and down-regulated the expression of bcl-2.ConclusionsExtrogenous H2S promotes the apoptosis in human uterine leiomyoma cells,which is related with the activation of ATP-sensitive K + channels by H2S. |
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