| 蒋红艳，杨林，欧阳贵，谢小明，王伟.IL-10-HGF融合基因对肝星状细胞LX-2增殖的抑制作用.[J].中南医学科学杂志.,2013,41(3):233-237. |
| IL-10-HGF融合基因对肝星状细胞LX-2增殖的抑制作用 |
| The Proliferation of Hepatic Stellate Cell LX-2 Inhibited by Human Interleukin-10 and Human Hepatocyte Growth Factor Fused Gene |
| 投稿时间：2012-12-12 |
| DOI： |
| 中文关键词: 人白介素10 人肝细胞生长因子 融合基因 肝星状细胞LX-2 |
| 英文关键词:human interleukin-10 human hepatocyte growth factor fused gene hepatic stellate cell LX-2 |
| 基金项目:湖南省卫生厅课题（B2008-059）. |
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| 中文摘要: |
| 目的探讨人白介素-10（IL-10）与人肝细胞生长因子（HGF）融合基因对肝星状细胞LX-2增殖的抑制作用。方法采用PCR方法，分别构建pcDNA3.1-flag-IL-10、pcDNA3.1-flag-HGF与pcDNA3.1-flag-IL-10-HGF真核表达载体，瞬时转染肝星状细胞LX-2；利用RT-PCR和Western blot检测LX-2细胞中IL-10、HGF的表达；选用MTT法检测转染24、48和72 h 后LX-2细胞的增殖情况。结果成功构建了IL-10-HGF融合基因真核表达载体pcDNA3.1-flag-IL-10-HGF。与pcDNA3.1-flag-IL-10和pcDNA3.1-flag-HGF组比较，pcDNA3.1-flag-IL-10-HGF融合组48 h和72 h LX-2细胞数减少（n=3，P＜0.05）。 结论IL-10-HGF融合基因可有效抑制肝星状细胞LX-2的增殖。 |
| 英文摘要: |
| ObjectiveThe study investigated the action of human interleukin-10 and human hepatocyte growth factor fused gene for the proliferation of cells LX-2.MethodsThe eukaryotic expression vector of pcDNA3.1-flag-IL-10,pcDNA3.1-flag-HGF and pcDNA3.1-flag-IL-10-HGF were constructed by PCR.They were transiently transfected with hepatic stellate cell LX-2.Then,the expression of human interleukin-10 and human hepatocyte growth factor were detected by RT-PCR and Western blot.The proliferation of LX-2 cells was checked by MTT method after transfected 24h,48h and 72h.ResultsThe eukaryotic expression vector pcDNA3.1-flag -IL-10-HGF including human interleukin-10 and human hepatocyte growth factor fused gene was successfully constructed.Compared with the groups of IL-10 and HGF,the LX-2 cell number were decreased in IL-10-HGF fused gene group after transfected 48h and 72h (n=3,P＜0.05).ConclusionThe IL-10-HGF fused gene can suppress the proliferation of hepatic stellate cell LX-2. |
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