| 赵国军, 汤石林,田国平,欧阳新平,吕运成, 何平平, 姚峰，唐艳艳,吴剑锋,王甲林,张敏,唐朝克.PDTC对ox-LDL诱导的THP-1巨噬细胞脂质蓄积和胆固醇流出的影响.[J].中南医学科学杂志.,2013,41(3):225-228. |
| PDTC对ox-LDL诱导的THP-1巨噬细胞脂质蓄积和胆固醇流出的影响 |
| Effect of PDTC on Lipid Accumulation and Cholesterol Efflux in THP-1 Macrophages Induced by ox-LDL |
| 投稿时间：2013-01-04 |
| DOI： |
| 中文关键词: 二硫代氨基甲酸吡咯烷 THP-1巨噬细胞 脂质蓄积 胆固醇流出 |
| 英文关键词:PDTC THP-1 macrophages lipid accumulation cholesterol efflux |
| 基金项目:国家自然科学基金资助项目（81200218,81170278,81070220），衡阳市科技局课题(2011KJ11). |
| 作者 | 单位 | | 赵国军1，2, 汤石林1,田国平1,欧阳新平1,吕运成1, 何平平1, 姚峰1，唐艳艳1,吴剑锋1,王甲林1,张敏1,唐朝克1 | 1.南华大学心血管病研究所 动脉硬化湖南省重点实验室 生命科学研究中心，湖南 衡阳 421001；2.南华大学医学院组织学与胚胎学教研室 |
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| 中文摘要: |
| 目的研究核因子-κB(NF-κB)抑制剂二硫代氨基甲酸吡咯烷（PDTC）对THP-1巨噬细胞脂质蓄积和胆固醇流出的影响。方法 60 nmol/L PMA孵育THP-1巨噬细胞24 h后，分为3组：对照组、氧化型低密度脂蛋白（ox-LDL）组（100 μg/mL）和PDTC组（100 μg/mL ox-LDL+50 μmol/L PDTC）。用油红O染色观察细胞内脂质蓄积情况，高效液相色谱分析法检测细胞内胆固醇水平，用液体闪烁计数法观察细胞内胆固醇的流出。 结果 PDTC抑制ox-LDL诱导的THP-1巨噬细胞脂质蓄积，明显降低细胞内总胆固醇、游离胆固醇和胆固醇酯的含量，增加载脂蛋白A-Ⅰ(apoA-Ⅰ)介导的胆固醇流出，但对HDL介导的胆固醇流出无明显影响。结论 PDTC抑制ox-LDL诱导的THP-1巨噬细胞脂质蓄积，促进apoA-Ⅰ介导的胆固醇流出。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of NF-κB inhibitor pyrrolidinedithiocarbamate (PDTC) on lipid accumulation and cholesterol efflux in THP-1 macrophages induced by ox-LDL. MethodsHuman THP-1 macrophage was induced using phorbol-12-myristate acetate (PMA,160 nmol/L) for 24 h.Then cells were divided into three groups:control group,ox-LDL group (100 μg/mL) and PDTC group (100 μg/mL ox-LDL+50 μmol/L PDTC).Oil red O staining was used to observe the intracellular lipid droplets.Cellular cholesterol was determined by high performance liquid chromatography analysis.Cholesterol efflux was determined by liquid scintillation counting. ResultsPDTC inhibited the lipid accumulation in THP-1 macrophage induced by ox-LDL.Cellular total cholesterol,free cholesterol and cholesterol ester were decreased by application of PDTC.PDTC increased apoA-Ⅰ mediated cholesterol efflux and had no significant effect on HDL-mediated cholesterol efflux. ConclusionPDTC inhibits lipid accumulation induced by ox-LDL and increases apoA-Ⅰ mediated cholesterol efflux in THP-1 macrophage. |
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