| 朱翠明，余敏君，曾焱华，游晓星，吴移谋.穿透支原体P35蛋白细胞粘附活性研究.[J].中南医学科学杂志.,2013,41(3):217-220. |
| 穿透支原体P35蛋白细胞粘附活性研究 |
| Studies on Cyto-adherence of the Mycoplasma Penetrans P35 |
| 投稿时间：2013-02-27 |
| DOI： |
| 中文关键词: 穿透支原体 P35蛋白 细胞粘附 |
| 英文关键词:Mycoplasma penetrans P35 adherence |
| 基金项目:国家自然科学基金（81072418/H1005）和湖南省教育厅重点实验室（2012年）项目. |
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| 中文摘要: |
| 目的研究穿透支原体（Mpe）P35蛋白的细胞粘附活性。方法IPTG诱导pQE31/p35原核表达载体在E.coli 中表达，用Ni-NTA Spin亲和纯化，Western blot鉴定表达产物。通过间接免疫荧光试验（IFA）、竞争抑制试验研究rP35对HeLa细胞的粘附活性。结果pQE31/p35在E.coli 中表达出分子量约35 kDa的蛋白质，Western blot鉴定其为特异性rP35蛋白。IFA发现rP35对HeLa细胞无明显的粘附活性；竞争抑制试验表明rP35不能抑制Mpe粘附HeLa细胞。结论P35可能不是Mpe的主要粘附分子。 |
| 英文摘要: |
| ObjectiveTo study the adherence of M.penetrans 35kDa Lipid associated membrane protein(P35).MethodspQE31/p35,a prokaryotic expression recombinant,was constructed.Expression of recombinant P35 protein(rP35) was induced by IPTG in E.coli.rP35 purified with Ni-NTA Spin Kit was analyzed by Western blot.Indirect immunofluorescence assay (IFA) and competitive inhibition test were used to analyze the adherence of rP35 to HeLa cells.ResultsrP35 fusion protein with a calculated molecular mass of 35kDa was expressed in E.coli.IFA showed that rP35 could not adhere to HeLa cells.The competitive inhibition test also showed that rP35 was not able to inhibit M.penetrans adhering to HeLa cells.ConclusionProbably P35 is not the main adhesin of M.penetrans. |
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