| 邹涛,王婧,谭琰,何爱桃,杨慧仙,张朝晖.过表达KLF4对RAW264.7巨噬细胞ABCA1表达和胆固醇蓄积的影响.[J].中南医学科学杂志.,2013,41(2):127-130. |
| 过表达KLF4对RAW264.7巨噬细胞ABCA1表达和胆固醇蓄积的影响 |
| Effect of KLF4 Overexpression on Cholesterol Accumulation and Expression of ABCA1 in RAW264.7 |
| 投稿时间：2012-09-18 |
| DOI： |
| 中文关键词: KLF4 泡沫细胞 ATP结合盒转运蛋白A1 动脉粥样硬化 |
| 英文关键词:KLF4 foam cells ATP binding cassette transporter A1 Atherosclerosis |
| 基金项目:衡阳市科学技术发展计划项目（2010KJ15） |
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| 中文摘要: |
| 目的观察KLF4过表达对小鼠巨噬细胞(RAW264.7)胆固醇蓄积的影响及对ATP结合盒转运蛋白A1(ABCA1)表达的影响。方法构建KLF4过表达的RAW264.7细胞株。将细胞分为三组:对照组、ox-LDL组、KLF4过表达+ox-LDL组,后两组加入终浓度为30 μg/mL的ox-LDL处理24 h。分别运用油红O染色观察细胞质脂滴变化，高效液相色谱法（HPLC）检测细胞内总胆固醇(TC)和游离胆固醇(FC)含量，Western blot法检测各组细胞ABCA1及KLF4蛋白表达。结果泡沫细胞模型组的细胞内存在大量的红色脂滴，KLF4及ABCA1蛋白表达及胆固醇含量较对照组升高；KLF4过表达细胞经ox-LDL处理后，细胞内脂滴较模型组明显减少，胆固醇含量下降，ABCA1蛋白表达进一步升高。结论KLF4可上调巨噬细胞ABCA1的表达，抑制ox-LDL诱导的RAW264.7巨噬细胞胆固醇蓄积。 |
| 英文摘要: |
| ObjectiveTo investigate cholesterol accumulation and expression of ATP binding cassette transporter A1(ABCA1) in RAW264.7 affected by Kruppel-like factor 4（KLF4）.MethodsStable subline of RAW264.7 cells over-expressed KLF4 were established.RAW264.7cells were induced into foam cells by 30 μg/ml oxidized low density lipoprotein （ox-LDL）for 24 h.Lipid droplets in cytoplasm were observed by Red oil O staining.The cellular contents of total cholesterol ( TC) and free cholesterol ( FC) were examined by high performance liquid chromatography （HPLC）.The protein expression of ABCA1 and KLF4 were detected by Western blot.ResultsIn model group，Oil red O-staining showed cells were filled with lipid droplet and the expression of ABCA1 and KLF4 increased compared the control group.Compared with the model group，lipid droplets and the contents of FC and CE were decreased in the KLF4 over -expressed cell，and the protein expression of ABCA1 increased.ConclusionKLF4 could up-regulate the expression of ABCA1 and inhibit the lipid accumulation induced by ox-LDL in RAW 264.7 macrophage-derived foam cells. |
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