| 敬开权，曾荣，顾洪丰，陶润知，杨湘怡，聂亚雄.慢性寒冷应激对高血压大鼠血浆及脑组织中ET-1和CGRP表达的影响.[J].中南医学科学杂志.,2013,41(2):118-123. |
| 慢性寒冷应激对高血压大鼠血浆及脑组织中ET-1和CGRP表达的影响 |
| Influence of Chronic Cold Stress on Expression of ET-1,CGRP in Plasma and Brain Tissue of Renovascular Hypertensive Rats |
| 投稿时间：2012-11-19 |
| DOI： |
| 中文关键词: 慢性寒冷应激 肾血管性高血压大鼠 内皮素-1 降钙素基因相关肽 脑卒中 |
| 英文关键词:Chronic cold stress Renovascular hypertensive rats ET-1 CGRP Stroke |
| 基金项目:湖南省自然科学基金（08JJ3036） |
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| 中文摘要: |
| 目的观察慢性寒冷应激对肾血管性高血压大鼠（RHR）血浆及脑组织中内皮素-1（ET-1）和降钙素基因相关肽（CGRP）表达的影响，并探讨内皮素-1（ET-1）和降钙素基因相关肽（CGRP）与高血压脑卒中发生之间的关系。方法将造模成功后的RHR与对照组大鼠（正常血压）各分为慢性寒冷应激组与非应激组，在慢性寒冷应激8周后处死大鼠，ELISA法检测血浆中ET-1和CGRP的表达，免疫组化法检测脑组织中ET-1和CGRP的表达。结果与RHR非应激组比较，RHR应激组大鼠血液及脑组织中ET-1的表达明显升高，CGRP表达明显下降，差异均有显著性(P<0.05)；与对照非应激组比较，对照应激组大鼠血液及脑组织中ET-1的表达明显升高，CGRP表达也明显升高，差异均有显著性(P<0.05)。结论慢性寒冷应激能打破RHR体内ET-1与CGRP的平衡，这可能是寒冷应激促使脑卒中发生的病理生理学机制之一。 |
| 英文摘要: |
| ObjectiveTo observe the influence of chronic cold stress on expression of endothelium-1 (ET-1) and calcitonin gene related peptide (CGRP) in plasma and brain tissue of renovascular hypertensive rats (RHR),and to investigate the relationship between ET-1,CGRP and hypertensive stroke. Method The wistar rats were divided into two groups: renovascular hypertensive rats（RHR）group and control group (normal blood pressure rats). Each group was further subdivided into two groups: chronic cold stress group and non-chronic cold stress group. At the 8th week of chronic cold stress all subgroups rats were sacrificed,plasma was saved to measure the expression of ET-1 and CGRP by Enzyme - linked immunosorbent assay（ELISA）and brain tissue was collected to measure the expression of ET-1 and CGRP by immunohistochemistry. Results In RHR chronic cold stress group,ET-1 was significantly higher while CGRP was significantly lower in plasma and brain tissue compared with the RHR non-chronic cold stress group (P < 0.05). Compared with the control non- chronic cold stress group，ET-1 and CGRP were both significantly higher in plasma and brain tissue of control chronic cold stress group. Conclusion Chronic cold stress can damage the balance of ET-1 and CGRP of HRH,which is possibly related to the pathological damage of cerebrovascular and hypertensive stroke. |
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